Talk:20.109(S10):Aptamer binding assay (Day7)

Binding Data Files

Group	T/R zip data file	W/F zip data file
Red	Media:S10_M1D7_TR-Red.zip	Media:S10_M1D7_WF-Red.zip
Orange	Media:S10_M1D7_TR-Orange.zip	Media:S10_M1D7_WF-Orange.zip
Yellow	Media:S10_M1D7_TR-Yellow.zip	Media:S10_M1D7_WF-Yellow.zip
Green	Media:S10_M1D7_TR-Green.zip	Media:S10_M1D7_WF-Green.zip
Blue	Media:S10_M1D7_TR-Blue.zip	Media:S10_M1D7_WF-Blue.zip
Pink	Media:S10_M1D7_TR-Pink.zip	Media:S10_M1D7_WF-Pink.zip
Purple	Media:S10_M1D7_TR-Purple.zip	Media:S10_M1D7_WF-Purple.zip

Binding Data Results Summary

T/R

Group	Corrected A405 values*	Pre % 8-12: expected, measured	Post % 8-12: fewer washes, more washes (measured)	Additional comments
Syntax sample	0.1; 0.2; 0.3 0.4; 0.5; 0.6	10%, 12%	40%, 50%	We used Grey group's 6-5 because we didn't have any.
red	0.278; 0.281; 0.0.298; 0.299; 0.35, 0.377	50% ; 51.4%	117%; 127%	We had to use extra '6-5' because we ran out of it. Our results came in harmony with expected. We had peak shifts. There was something definitely wrong with our '8-12' pre absorbance, which was abnormally low so we relied heavily on the correcting method to get a reasonable value for it.
orange	0.181392157 0.182 0.236803922 0.18354902 0.256019608 0.281509804	2%; 3.3696%	4 washes: 134.8675%; 24 washes: 181.1195%	Only used .1nmols each for the binding assay samples Normalized Data: Media:NormalizedAbsorbance.png Raw Data: Media:RawAbsorbance.png Peak shifts are kind of as expected. 8-12 and post mixtures have peaks at a higher wavelength than 6-5 and heme, as expected. However, post mixtures have higher absorbance relative to 8-12 pre absorbance at peaks than expected.
Yellow
Green	0.216, 0.217, 0.292, 0.227, 0.258, 0.283	10%; 13.333%	4 washes: 65.667%; 24 washes: 88.000%	File:20.109 assay graphs.pdf Due to the corrections, the heme and 6-5 aptamer horizontal peaks are a little off; the 6-5 aptamer graph should be shifted up from the heme graph at low absorbances, but instead, it's simply lined up with the heme graph. The other graphs seem consistent with the fact that 8-12 graph should be higher horizontally than the 6-5 graph, with the heights increasing in the following order: mix 10% pre, mix 10% post 4x, post 24x, and 8-12 aptamer.
Blue
Pink
Purple	0.254; 0.259; 0.3559 0.2971; N/A; N/A	50%, 41.5%	N/A	We did not obtain proper data for the mixes post-wash, unfortunately. Also, our 8-12 "pre" sample came from the Blue group. <3 The horizontal and vertical shifts for the samples we did have all appear consistent with our expected results. Corrected Data: Media:Yuan-mod1spec-CORRECTED.png Raw Data: Media:Yuan-mod1spec.png

* Be sure to post the values you got after baseline correction, not the raw data (if your curves didn't line up)!
* Order: heme, 6-5, 8-12, "pre" mix, post low wash, post high wash
* Recommended syntax for A405 column: "#1 ; #2; #3 <br> #4; #5; #6"

W/F

Group	Corrected A405 values*	Pre % 8-12: expected, measured	Post % 8-12: fewer washes, more washes (measured)	Additional comments
Syntax sample	0.1; 0.2; 0.3 0.4; 0.5; 0.6	10%, 12%	40%, 50%	We used Grey group's 6-5 because we didn't have any.
Red	0.260;0.252;0.323;0.262;0.311;0.30888	10%, 3.5%	8 Washes 81%, 16 Washes 78%	Trying to figure out what went wrong with premix ratios.
Orange
Yellow
Green	0.248; 0.245; 0.313; 0.253; 0.289; 0.287	10%, 12%	8 Washes 64%, 16 Washes 61%
Blue
Pink
Purple

* Be sure to post the values you got after baseline correction, not the raw data (if your curves didn't line up)!
* Order: heme, 6-5, 8-12, "pre" mix, post low wash, post high wash
* Recommended syntax for A405 column: "#1 ; #2; #3 <br> #4; #5; #6"