Talk:20.109(S10):Induce protein and evaluate DNA (Day5): Difference between revisions
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|Yellow | |Yellow | ||
| | |.152 | ||
| | |.151 | ||
| | |.146 | ||
| | |All pellets were slightly yellow, but we decided to place them in the shaker at room temp. overnight. | ||
|- | |- | ||
|Green | |Green | ||
Revision as of 14:45, 30 March 2010
TR O/N Cultures and Gels
Below you should list the OD600 values of the induced cultures (at 1:10 dilutions), as well as your pellet observations. The teaching faculty will fill in this information for the cultures needing to be grown overnight.
| Group | OD WT | OD M124S | OD Chosen mutant | Pellets yellow? |
|---|---|---|---|---|
| Red | ||||
| Orange | ||||
| Yellow | .152 | .151 | .146 | All pellets were slightly yellow, but we decided to place them in the shaker at room temp. overnight. |
| Green | ||||
| Blue | 0.169 | 0.205 | D129P - #1 | Slightly yellow except for M124S sample |
| Pink | ||||
| Purple |
Gels
WF O/N Cultures and Gels
Below you should list the OD600 values of the induced cultures (at 1:10 dilutions), as well as your pellet observations. The teaching faculty will fill in this information for the cultures needing to be grown overnight.
| Group | OD WT | OD M124S | OD Chosen mutant | Pellets yellow? |
|---|---|---|---|---|
| Red | ||||
| Orange | ||||
| Yellow | ||||
| Green | ||||
| Blue | ||||
| Pink | ||||
| Purple |
