Talk:20.109(S10):Testing cell viability (Day3)

T/R schedule

Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.

Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.

Microscope time	Arrival time (at latest!)	Team colour
2:05-2:30	1:05	Red
2:30-2:55	1:20	Yellow
2:55-3:20	1:45	Blue
3:20-3:45	2:10	Orange
3:45-4:10	2:35	Green
4:10-4:35	3:00	Purple
4:35-5:00	3:25	Pink

This image was taken from the cross section of a bead at biological pH. The concentration of cells as well as the ratio of dead (red) to live (green) cells is representative of all frames observed. The distribution of cells was uniform at different layers of the bead. This image was taken from the surface a bead at pH 8. The concentration of cells as well as the ratio of dead (red) to live (green) cells is representative of all frames observed. The distribution of cells was uniform at different layers of the bead. There does not appear to be a significant difference in viability between high and low pH, but the density of cells in high pH was approximately 5 x lower than in biological pH.

Team colour
Red with Chelator
Red without Chelator
Yellow with biological pH (7.2-7.4)
Yellow with pH 8.0
Blue	Orange	Green	Purple	Pink

Start time	Team colour(s)
first (is the worst)	Yellow,Green,Red
second (is the best)	Purple,Orange, Blue
third (is ---)	Pink

W/F schedule

Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.

Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.

Microscope time	Arrival time (at latest!)	Team colour
2:05-2:30	1:05	Orange
2:30-2:55	1:20	Red
2:55-3:20	1:45	Yellow
3:20-3:45	2:10	Purple
3:45-4:10	2:35	Pink
4:10-4:35	3:00	Green
4:35-5:00	3:25	Blue

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