Talk:20.109(S10):Testing cell viability (Day3): Difference between revisions
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Revision as of 12:10, 23 April 2010
T/R schedule
Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.
Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.
| Microscope time | Arrival time (at latest!) | Team colour |
| 2:05-2:30 | 1:05 | Red |
| 2:30-2:55 | 1:20 | Yellow |
| 2:55-3:20 | 1:45 | Blue |
| 3:20-3:45 | 2:10 | Orange |
| 3:45-4:10 | 2:35 | Green |
| 4:10-4:35 | 3:00 | Purple |
| 4:35-5:00 | 3:25 | Pink |
| Team colour | |
| Red (a)
|
Red (b)
|
| Fluorescence Assay is used to tack and compare cell viability. At a magnification of 10X, the viability of the cells in beads without chelator (a) and with chelator (b) were relatively similar. | |
| Yellow with biological pH (7.2-7.4)
| |
| This image was taken from the cross section of a bead at biological pH.
The concentration of cells as well as the ratio of dead (red) to live (green) cells is representative of all frames observed. The distribution of cells was uniform at different layers of the bead. | |
| Yellow with pH 8.0
| |
| This image was taken from the surface a bead at pH 8. The concentration of cells
as well as the ratio of dead (red) to live (green) cells is representative of all frames observed. The distribution of cells was uniform at different layers of the bead. There does not appear to be a significant difference in viability between high and low pH, but the density of cells in high pH was approximately 5 x lower than in biological pH. | |
| Blue
| |
| Orange
| |
| Green
File:LIVE DEAD FIGURE.pdf Sorry, I actually don't know how to upload it directly onto the page... | |
| Purple
| |
| Pink |
| Start time | Team colour(s) |
| first (is the worst) | Yellow,Green,Red |
| second (is the best) | Purple,Orange, Blue |
| third (is ---) | Pink |
W/F schedule
Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.
Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.
| Microscope time | Arrival time (at latest!) | Team colour |
| 2:05-2:30 | 1:05 | Orange |
| 2:30-2:55 | 1:20 | Red |
| 2:55-3:20 | 1:45 | Yellow |
| 3:20-3:45 | 2:10 | Purple |
| 3:45-4:10 | 2:35 | Pink |
| 4:10-4:35 | 3:00 | Green |
| 4:35-5:00 | 3:25 | Blue |
[[[[]]]]
| Team colour |
| Red (a) |
| Yellow with biological pH (7.2-7.4) |
| Blue |
| File:F).pdf |
| Green
File:LIVE DEAD FIGURE.pdf Sorry, I actually don't know how to upload it directly onto the page... |
| Purple
|
| Pink |
