Talk:20.109(S11):Aptamer binding assay (Day7): Difference between revisions
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|We used Purple group's mixture | |We used Purple group's mixture | ||
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| | |Pink | ||
| | |0.295, 0.547, 0.546 <br> 0.547, 0.553, 0.56 | ||
| | |%2, n/a | ||
| | |Washes conducted: 4 and 24 | ||
| | |Our graph shows no shift in absorbance peaks and so there is not a significant enough difference in absorbance values to calculate the actual percentages of pre, and post washes. This may be due to experimental error or | ||
also indicate that having an initial percentage of 2% 8-12 is too low to start the SELEX process with and we would ideally want to use more | |||
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<br style="clear:both;"/> | <br style="clear:both;"/> | ||
[[Media:[[Media:Example.ogg]]]] | |||
===W/F=== | ===W/F=== | ||
Revision as of 13:47, 5 March 2011
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Binding Data Files
| Group | T/R zip data file | W/F zip data file |
|---|---|---|
| Red | Media:S11_M1D7_TR-Red.zip | Media:S11_M1D7_WF-Red.zip |
| Orange | Media:S11_M1D7_TR-Orange.zip | Media:S11_M1D7_WF-Orange.zip |
| Yellow | Media:S11_M1D7_TR-Yellow.zip | Media:S11_M1D7_WF-Yellow.zip |
| Green | Media:S11_M1D7_TR-Green.zip | Media:S11_M1D7_WF-Green.zip |
| Blue | Media:S11_M1D7_TR-Blue.zip | Media:S11_M1D7_WF-Blue.zip |
| Pink | Media:S11_M1D7_TR-Pink.zip | Media:S11_M1D7_WF-Pink.zip |
| Purple | Media:S11_M1D7_TR-Purple.zip | Media:S11_M1D7_WF-Purple.zip |
Binding Data Results Summary
T/R
* Be sure to post the values you got after baseline correction, not the raw data (if your curves didn't line up)!
* Order: heme, 6-5, 8-12, "pre" mix, post low wash, post high wash
* Recommended syntax for A405 column: "#1 ; #2; #3 <br> #4; #5; #6"
| Group | Corrected A405 values* | Pre % 8-12: expected, measured | Post % 8-12: fewer washes, more washes (measured) | Additional comments |
|---|---|---|---|---|
| Syntax sample | 0.1; 0.2; 0.3 0.4; 0.5; 0.6 |
10%, 12% | 40%, 50% | We used Grey group's 6-5 because we didn't have any. |
| Green | 0.669; 0.724; 0.713 0.709; 0.786; 0.772 |
We used Purple group's mixture | ||
| Pink | 0.295, 0.547, 0.546 0.547, 0.553, 0.56 |
%2, n/a | Washes conducted: 4 and 24 | Our graph shows no shift in absorbance peaks and so there is not a significant enough difference in absorbance values to calculate the actual percentages of pre, and post washes. This may be due to experimental error or
also indicate that having an initial percentage of 2% 8-12 is too low to start the SELEX process with and we would ideally want to use more |
[[Media:Media:Example.ogg]]
W/F
* Be sure to post the values you got after baseline correction, not the raw data (if your curves didn't line up)!
* Order: heme, 6-5, 8-12, "pre" mix, post low wash, post high wash
* Recommended syntax for A405 column: "#1 ; #2; #3 <br> #4; #5; #6"
| Group | Corrected A405 values* | Pre % 8-12: expected, measured | Post % 8-12: fewer washes, more washes (measured) | Additional comments |
|---|---|---|---|---|
| Syntax sample | 0.1; 0.2; 0.3 0.4; 0.5; 0.6 |
10%, 12% | 40%, 50% | We used Grey group's 6-5 because we didn't have any. |
| Green | 0.655; 0.655; 0.737 0.656; 0.668; 0.681 |
0.25%, 1.21% | 16.3%, 32.3% | |
| Blue | 0.649; 0.753; 0.841 0.716; 0.767; 0.761 |
10%, 10.71% | 47.14%; 42.86% | We used the TTh Orange group's 6-5 & mixtures. |
| Orange | 0.649; 0.638; 0.744 0.632; 0.637; 0.736 |
2%, <0% ? | 0, 92.1% | Pre mixture measured lower than 6-5 pre. Due to experimental error, 2 wash measured lower than 6-5 pre. |
