Talk:20.109(S11):Aptamer binding assay (Day7): Difference between revisions
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|Our graph shows no shift in absorbance peaks and so there is not a significant enough difference in absorbance values to calculate the actual percentages of pre, and post washes. This may be due to experimental error or | |Our graph shows no shift in absorbance peaks and so there is not a significant enough difference in absorbance values to calculate the actual percentages of pre, and post washes. This may be due to experimental error or | ||
also indicate that having an initial percentage of 2% 8-12 is too low to start the SELEX process with and we would ideally want to use more | also indicate that having an initial percentage of 2% 8-12 is too low to start the SELEX process with and we would ideally want to use more | ||
|[[Image:pinkcurve.jpg]] | |||
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Revision as of 13:50, 5 March 2011
Binding Data Files
Group | T/R zip data file | W/F zip data file |
---|---|---|
Red | Media:S11_M1D7_TR-Red.zip | Media:S11_M1D7_WF-Red.zip |
Orange | Media:S11_M1D7_TR-Orange.zip | Media:S11_M1D7_WF-Orange.zip |
Yellow | Media:S11_M1D7_TR-Yellow.zip | Media:S11_M1D7_WF-Yellow.zip |
Green | Media:S11_M1D7_TR-Green.zip | Media:S11_M1D7_WF-Green.zip |
Blue | Media:S11_M1D7_TR-Blue.zip | Media:S11_M1D7_WF-Blue.zip |
Pink | Media:S11_M1D7_TR-Pink.zip | Media:S11_M1D7_WF-Pink.zip |
Purple | Media:S11_M1D7_TR-Purple.zip | Media:S11_M1D7_WF-Purple.zip |
Binding Data Results Summary
T/R
* Be sure to post the values you got after baseline correction, not the raw data (if your curves didn't line up)!
* Order: heme, 6-5, 8-12, "pre" mix, post low wash, post high wash
* Recommended syntax for A405 column: "#1 ; #2; #3 <br> #4; #5; #6"
[[Media:Media:Example.ogg]]
W/F
* Be sure to post the values you got after baseline correction, not the raw data (if your curves didn't line up)!
* Order: heme, 6-5, 8-12, "pre" mix, post low wash, post high wash
* Recommended syntax for A405 column: "#1 ; #2; #3 <br> #4; #5; #6"
Group | Corrected A405 values* | Pre % 8-12: expected, measured | Post % 8-12: fewer washes, more washes (measured) | Additional comments |
---|---|---|---|---|
Syntax sample | 0.1; 0.2; 0.3 0.4; 0.5; 0.6 |
10%, 12% | 40%, 50% | We used Grey group's 6-5 because we didn't have any. |
Green | 0.655; 0.655; 0.737 0.656; 0.668; 0.681 |
0.25%, 1.21% | 16.3%, 32.3% | |
Blue | 0.649; 0.753; 0.841 0.716; 0.767; 0.761 |
10%, 10.71% | 47.14%; 42.86% | We used the TTh Orange group's 6-5 & mixtures. |
Orange | 0.649; 0.638; 0.744 0.632; 0.637; 0.736 |
2%, <0% ? | 0, 92.1% | Pre mixture measured lower than 6-5 pre. Due to experimental error, 2 wash measured lower than 6-5 pre. |