Talk:20.109(S11):Aptamer binding assay (Day7)

Binding Data Files

Group	T/R zip data file	W/F zip data file
Red	Media:S11_M1D7_TR-Red.zip	Media:S11_M1D7_WF-Red.zip
Orange	Media:S11_M1D7_TR-Orange.zip	Media:S11_M1D7_WF-Orange.zip
Yellow	Media:S11_M1D7_TR-Yellow.zip	Media:S11_M1D7_WF-Yellow.zip
Green	Media:S11_M1D7_TR-Green.zip	Media:S11_M1D7_WF-Green.zip
Blue	Media:S11_M1D7_TR-Blue.zip	Media:S11_M1D7_WF-Blue.zip
Pink	Media:S11_M1D7_TR-Pink.zip	Media:S11_M1D7_WF-Pink.zip
Purple	Media:S11_M1D7_TR-Purple.zip	Media:S11_M1D7_WF-Purple.zip

Binding Data Results Summary

T/R

* Be sure to post the values you got after baseline correction, not the raw data (if your curves didn't line up)!
* Order: heme, 6-5, 8-12, "pre" mix, post low wash, post high wash
* Recommended syntax for A405 column: "#1 ; #2; #3 <br> #4; #5; #6"

Group	Corrected A405 values*	Pre % 8-12: expected, measured	Post % 8-12: fewer washes, more washes (measured)	Additional comments
Syntax sample	0.1; 0.2; 0.3 0.4; 0.5; 0.6	10%, 12%	40%, 50%	We used Grey group's 6-5 because we didn't have any.
Green	0.669; 0.724; 0.713 0.709; 0.786; 0.772			We used Purple group's mixture
Pink	0.62, 0.55, 0.55 0.547, 0.553, 0.56	%2, n/a	Washes conducted: 4 and 24	Our graph shows no shift in absorbance peaks and so there is not a significant enough difference in absorbance values to calculate the actual percentages of pre, and post washes. This may be due to experimental error or also indicate that having an initial percentage of 2% 8-12 is too low to start the SELEX process with and we would ideally want to use more
Blue	0.0000 0.0800 0.0600 0.0840 0.0310 0.0150	2%	Washes:4 and 32	Our graph shows the shift in absorbance shown in the graph.
Purple	0.36549, 0.455, 0.393628 0.379314, 0.41143137, 0.416490196	10%, n/a	Washes conducted: 49.6% (for 8 washes), 57.8% (for 32 washes)	The percentages of the post- are higher after the 32 washes than in the 8 washes, suggesting that more washes can be done to obtain greater concentrations of 8-12 collected. The 8-12 pre curve is vertically higher than the 6-5 pre curve, which is consistent with expections. However, the post- curves were expected to be between the the two curves, but they turned out to exceed the 8-12 pre- curve.

[[Media:Media:Example.ogg]]

W/F

* Be sure to post the values you got after baseline correction, not the raw data (if your curves didn't line up)!
* Order: heme, 6-5, 8-12, "pre" mix, post low wash, post high wash
* Recommended syntax for A405 column: "#1 ; #2; #3 <br> #4; #5; #6"

Group	Corrected A405 values*	Pre % 8-12: expected, measured	Post % 8-12: fewer washes, more washes (measured)	Additional comments
Syntax sample	0.1; 0.2; 0.3 0.4; 0.5; 0.6	10%, 12%	40%, 50%	We used Grey group's 6-5 because we didn't have any.
Green	0.655; 0.655; 0.737 0.656; 0.668; 0.681	0.25%, 1.21%	16.3%, 32.3%
Blue	0.649; 0.753; 0.841 0.716; 0.767; 0.761	10%, 10.71%	47.14%; 42.86%	We used the TTh Orange group's 6-5 & mixtures.
Orange	0.649; 0.638; 0.744 0.632; 0.637; 0.736	2%, <0% ?	0, 92.1%	Pre mixture measured lower than 6-5 pre. Due to experimental error, 2 wash measured lower than 6-5 pre.
Purple	0.717; 0.729; 0.802 0.760; 0.780; 0.769	50%, 42.47%	69.86%, 54.79%	On Day 4, we used 6-5 and 8-12 from the orange, green, and faculty as well as some of ours, because after purification we didn't have enough of either one after purification. This could have caused our lower initial concentration.