Talk:20.109(S11):Aptamer binding assay (Day7): Difference between revisions
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Huayu Ding (talk | contribs) (→T/R) |
Jenny Cheng (talk | contribs) (→T/R) |
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|We used Purple group's mixture | |We used Purple group's mixture | ||
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Revision as of 15:41, 5 March 2011
Binding Data Files
Group | T/R zip data file | W/F zip data file |
---|---|---|
Red | Media:S11_M1D7_TR-Red.zip | Media:S11_M1D7_WF-Red.zip |
Orange | Media:S11_M1D7_TR-Orange.zip | Media:S11_M1D7_WF-Orange.zip |
Yellow | Media:S11_M1D7_TR-Yellow.zip | Media:S11_M1D7_WF-Yellow.zip |
Green | Media:S11_M1D7_TR-Green.zip | Media:S11_M1D7_WF-Green.zip |
Blue | Media:S11_M1D7_TR-Blue.zip | Media:S11_M1D7_WF-Blue.zip |
Pink | Media:S11_M1D7_TR-Pink.zip | Media:S11_M1D7_WF-Pink.zip |
Purple | Media:S11_M1D7_TR-Purple.zip | Media:S11_M1D7_WF-Purple.zip |
Binding Data Results Summary
T/R
* Be sure to post the values you got after baseline correction, not the raw data (if your curves didn't line up)!
* Order: heme, 6-5, 8-12, "pre" mix, post low wash, post high wash
* Recommended syntax for A405 column: "#1 ; #2; #3 <br> #4; #5; #6"
[[Media:Media:Example.ogg]]
W/F
* Be sure to post the values you got after baseline correction, not the raw data (if your curves didn't line up)!
* Order: heme, 6-5, 8-12, "pre" mix, post low wash, post high wash
* Recommended syntax for A405 column: "#1 ; #2; #3 <br> #4; #5; #6"
Group | Corrected A405 values* | Pre % 8-12: expected, measured | Post % 8-12: fewer washes, more washes (measured) | Additional comments |
---|---|---|---|---|
Syntax sample | 0.1; 0.2; 0.3 0.4; 0.5; 0.6 |
10%, 12% | 40%, 50% | We used Grey group's 6-5 because we didn't have any. |
Green | 0.655; 0.655; 0.737 0.656; 0.668; 0.681 |
0.25%, 1.21% | 16.3%, 32.3% | |
Blue | 0.649; 0.753; 0.841 0.716; 0.767; 0.761 |
10%, 10.71% | 47.14%; 42.86% | We used the TTh Orange group's 6-5 & mixtures. |
Orange | 0.649; 0.638; 0.744 0.632; 0.637; 0.736 |
2%, <0% ? | 0, 92.1% | Pre mixture measured lower than 6-5 pre. Due to experimental error, 2 wash measured lower than 6-5 pre. |
Purple | 0.717; 0.729; 0.802 0.760; 0.780; 0.769 |
50%, 42.47% | 69.86%, 54.79% | On Day 4, we used 6-5 and 8-12 from the orange, green, and faculty as well as some of ours, because after purification we didn't have enough of either one after purification. This could have caused our lower initial concentration. |