Talk:20.109(S11):Aptamer binding assay (Day7): Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Anirudh Arun (talk | contribs) (→W/F) |
Anirudh Arun (talk | contribs) (→W/F) |
||
Line 152: | Line 152: | ||
|0.25%, 1.21% | |0.25%, 1.21% | ||
|16.3%, 32.3% | |16.3%, 32.3% | ||
| | |||
|[[Image: M1D7Graph.jpg | thumb | 200px | Binding Curves GREEN]] | |[[Image: M1D7Graph.jpg | thumb | 200px | Binding Curves GREEN]] | ||
|- | |- |
Revision as of 12:03, 6 March 2011
Binding Data Files
Group | T/R zip data file | W/F zip data file |
---|---|---|
Red | Media:S11_M1D7_TR-Red.zip | Media:S11_M1D7_WF-Red.zip |
Orange | Media:S11_M1D7_TR-Orange.zip | Media:S11_M1D7_WF-Orange.zip |
Yellow | Media:S11_M1D7_TR-Yellow.zip | Media:S11_M1D7_WF-Yellow.zip |
Green | Media:S11_M1D7_TR-Green.zip | Media:S11_M1D7_WF-Green.zip |
Blue | Media:S11_M1D7_TR-Blue.zip | Media:S11_M1D7_WF-Blue.zip |
Pink | Media:S11_M1D7_TR-Pink.zip | Media:S11_M1D7_WF-Pink.zip |
Purple | Media:S11_M1D7_TR-Purple.zip | Media:S11_M1D7_WF-Purple.zip |
Binding Data Results Summary
T/R
* Be sure to post the values you got after baseline correction, not the raw data (if your curves didn't line up)!
* Order: heme, 6-5, 8-12, "pre" mix, post low wash, post high wash
* Recommended syntax for A405 column: "#1 ; #2; #3 <br> #4; #5; #6"
[[Media:Media:Example.ogg]]
Media:Example.ogg
W/F
* Be sure to post the values you got after baseline correction, not the raw data (if your curves didn't line up)!
* Order: heme, 6-5, 8-12, "pre" mix, post low wash, post high wash
* Recommended syntax for A405 column: "#1 ; #2; #3 <br> #4; #5; #6"
Group | Corrected A405 values* | Pre % 8-12: expected, measured | Post % 8-12: fewer washes, more washes (measured) | Additional comments | |
---|---|---|---|---|---|
Syntax sample | 0.1; 0.2; 0.3 0.4; 0.5; 0.6 |
10%, 12% | 40%, 50% | We used Grey group's 6-5 because we didn't have any. | |
Green | 0.655; 0.655; 0.737 0.656; 0.668; 0.681 |
0.25%, 1.21% | 16.3%, 32.3% | ||
Blue | 0.753; 0.753; 0.833 0.765; 0.830; 0.816 |
10%, 15.72% | 2 washes: 96.05%; 34 washes: 78.53% | We used the T/R Orange group's 6-5 to make our 10% 8-12 mixtures. We used 6-5 (pre) as our base line for correction. | |
Orange | 0.649; 0.638; 0.744 0.632; 0.637; 0.736 |
2%, <0% ? | 0, 92.1% | Pre mixture measured lower than 6-5 pre. Due to experimental error, 2 wash measured lower than 6-5 pre. | |
Purple | 0.717; 0.729; 0.802 0.760; 0.780; 0.769 |
50%, 42.47% | 69.86%, 54.79% | On Day 4, we used 6-5 and 8-12 from the orange, green, and faculty as well as some of ours, because after purification we didn't have enough of either one after purification. This could have caused our lower initial concentration. | |
Red | 0.687; 0.687; 0.770 0.689; 0.734; 0.744 |
2%, 2.88% | 57.31%, 69.42% | We used 6-5 from orange, green, and faculty because our 6-5 concentration was too low. | |
Yellow | 0.653; 0.654; 0.751 0.709; 0.723; 0.733 |
50%, 56.27% | 70.68%, 81.62% | We only used our own RNA. | |