Talk:20.109(S11):Initiate cell culture (Day2)

Plan for Day 2

Group 1 should arrive by 1:05 pm at the latest and immediately go to the tissue culture room. When everyone has arrived, we will thaw your cells in the water bath. After you have finished your culture preparations (ideally by 3 pm), you can take a 10 minute break to refresh your minds, and then will take a short quiz.

Group 2 should arrive by 2:45 pm at the latest and will begin by taking the quiz. If all goes well, you will begin working in the tissue culture room at 3 pm. If your culture preparations that involve a special physical set-up, you can come earlier and work in the extra tissue culture hood.

Notebooks may be handed in by Friday the 15th at 5 pm for the T/R section, and by Wednesday the 20th at 5 pm for the W/F section. (Earlier is certainly welcome for W/F folks if you are not away for the holiday.)

T/R

Arrival time (at latest!)	1:05 pm	2:45 pm
Team colour 1	Pink	Green
Team colour 2	Purple
Team colour 3	Red
Team colour 4	Yellow

W/F

Arrival time (at latest!)	1:05 pm	2:45 pm
Team colour 1	Purple	Red
Team colour 2	Blue
Team colour 3	Orange
Team colour 4	Pink

Designs (T/R)

Team	Design
Yellow	We decided to test the effects of stiffness, through the use of low and high calcium concentrations, on the rate of dedifferentiation of chondrocytes. We expect there to be less dedifferentiation on stiffer alginate (high calcium concentration). Thus, we expect that the cells on a stiffer alginate will maintain a chondrocyte-like phenotype better than those on a less stiff surface, because similar results have been recorded using a smaller range of calcium concentration (Genes et al. 2003). We also plan to compare our results using medium viscosity alginate with those of Red and Orange, who are using low viscosity alginate.
Red	We predict the alginate in 200mM calcium will maintain chondrocyte phenotype better than alginate in 20mM calcium because the 200mM calcium forms a stiffer porous scaffold, allowing for the cells to form a better matrix