Talk:20.109(S11):Purify RNA and run affinity column (Day4): Difference between revisions
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Revision as of 12:12, 11 February 2011
Sign-up for column protocols
T/R
You and your partner should propose a protocol in the table below.
If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.
Protocol# | Group | 8-12 % | Wash # (partner 1) | Wash # (partner 2) | Note changes to plan here |
---|---|---|---|---|---|
0 | Sample | 5 | 5 | 15 | Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12. |
1 | Red | .5 | 4 | 24 | Starting with a lower 8-12 concentration. |
2 | Orange | 50 | 8 | 16 | Repeat high-percentage run because the decrease in enrichment with increasing number of washes intrigued us |
3 | Yellow | 1 | 4 | 24 | starting with a lower 8-12 concentration |
4 | Green | 50 | 4 | 24 | Repeating high-percentage run for statistical reliability. |
5 | Blue | 2 | 4 | 32 | Check to see at what limit does the trend of more washes contribute to the yield hold true. |
6 | Pink | 2 | 4 | 24 | Repeat from last year's experiment |
7 | Purple | 10 | 8 | 32 | Interested in seeing the effect of introducing greater number of washes |
W/F
You and your partner should propose a protocol in the table below.
If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.
Protocol# | Group | 8-12 % | Wash # (partner 1) | Wash # (partner 2) | Note changes to plan here |
---|---|---|---|---|---|
0 | Sample | 5 | 5 | 15 | Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12. |
1 | Red | 2 | 4 | 24 | |
2 | Orange | ||||
3 | Yellow | ||||
4 | Green | ||||
5 | Blue | ||||
6 | Pink | ||||
7 | Purple | 50 | 4 | 24 | repeating last year's experiment that did not actually have 50% |