Talk:20.109(S11):Purify RNA and run affinity column (Day4): Difference between revisions
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Revision as of 12:42, 10 February 2011
Sign-up for column protocols
T/R
You and your partner should propose a protocol in the table below.
If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.
| Protocol# | Group | 8-12 % | Wash # (partner 1) | Wash # (partner 2) | Note changes to plan here |
|---|---|---|---|---|---|
| 0 | Sample | 5 | 5 | 15 | Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12. |
| 1 | Red | .5 | 4 | 24 | Starting with a lower 8-12 concentration. |
| 2 | Orange | ||||
| 3 | Yellow | 1 | 4 | 24 | starting with a lower 8-12 concentration |
| 4 | Green | 50 | 4 | 24 | Repeating high-percentage run for statistical reliability. |
| 5 | Blue | 10 | 36 | More washes | |
| 6 | Pink | 2 | 4 | 24 | Repeat from last year's experiment |
| 7 | Purple | 10 | 8 | 32 | Interested in seeing the effect of introducing greater number of washes |
W/F
You and your partner should propose a protocol in the table below.
If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.
| Protocol# | Group | 8-12 % | Wash # (partner 1) | Wash # (partner 2) | Note changes to plan here |
|---|---|---|---|---|---|
| 0 | Sample | 5 | 5 | 15 | Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12. |
| 1 | Red | ||||
| 2 | Orange | ||||
| 3 | Yellow | ||||
| 4 | Green | ||||
| 5 | Blue | ||||
| 6 | Pink | ||||
| 7 | Purple |
