Talk:20.109(S11):Testing cell viability (Day3): Difference between revisions
Lauren Berry (talk | contribs) |
Jingxun Chen (talk | contribs) |
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|1:20 | |1:20 | ||
|Pink | |Pink | ||
|We tested a cell density of around 1e7 cells in 1.5% low viscosity and 2e6 in 1.5% medium viscosity. | |||
|[[Image:DSCN1501.JPG|thumb|center|Surface of 1.5% low viscosity bead.]] | |||
|[[Image:MedViscosity.JPG|thumb|center|Surface of 1.5% medium viscosity bead.]] | |||
|- | |- | ||
|2:55-3:20 | |2:55-3:20 | ||
|1:45 | |1:45 | ||
|Blue | |Blue | ||
|We tested the impact of different alginate viscosities on chondrocyte differentiation. Cell density was 1 million cells/mL in 1.5% low and 1.5% medium viscosity alginate. | |||
|[[Image:DSCN1515.jpg|thumb|center|Surface of 1.5% low viscosity bead.]] | |||
|[[Image:DSCN1511.jpg|thumb|center|Center of 1.5% medium viscosity bead.]] | |||
|- | |- | ||
|3:20-3:45 | |3:20-3:45 | ||
|2:10 | |2:10 | ||
|Green | |Green | ||
|We wanted to see if seeding a stem cell culture with chondrocytes could increase the rate of differentiation. Cell density was 3.6 million cells/mL in 1.5% low viscosity alginate. | |||
|[[Image:DSCN1524.jpg|thumb|center|Core of chondrocyte-only bead.]] | |||
[[Image:DSCN1520.jpg|thumb|center|Core/Edge of stem-cell only bead. The left, near the edge, has many dead cells, while the right, near the core, has mostly alive cells.]] | |||
|[[Image:DSCN1516.jpg|thumb|center|Core of 2:1 Chondrocyte:stem cell bead.]] | |||
|- | |- | ||
|3:45-4:10 | |3:45-4:10 | ||
|2:35 | |2:35 | ||
|Orange | |Orange | ||
|We were testing the influence of BMP-6 upon the differentiation of mesenchymal stem cells into chondrocytes. Cells were cultured in 1% medium-viscosity alginate. | |||
|[[Image:OrangeImageOne.jpg|thumb|center|Center of the control bead.]] | |||
|[[Image:OrangeImageTwo.jpg|thumb|center|Center of the BMP-6 exposed bead.]] | |||
|- | |- | ||
|4:10-4:35 | |4:10-4:35 | ||
|3:00 | |3:00 | ||
|Yellow | |Yellow | ||
|We tested chondrocytes on 1.5% medium-viscosity alginate prepared in regular control conditions, or with addition of 800 uM glycine, a major component of collagen. | |||
|[[Image:Mod3day3wfyellownoglycine.jpg|thumb|center|Center of the control (no glycine) bead.]] | |||
|[[Image:Mod3day3wfyellowglycine.JPG|thumb|center|Center of the bead under +glycine condition.]] | |||
|- | |- | ||
|4:35-5:00 | |4:35-5:00 | ||
|3:25 | |3:25 | ||
|Red | |Red | ||
|We tested chondrocytes on 1% medium-viscosity alginate prepared in either 20mM or 200mM CaCl2 by Live/Dead Fluorescence Assay. | |||
|[[Image:Talk_page_Ca_20mM_pic.jpg|thumb|center]] | |||
|[[Image:Talk_page_Ca_200mM_pic.jpg|thumb|center]] | |||
|- | |- | ||
|} | |} |
Latest revision as of 01:47, 5 May 2011
T/R schedule
Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.
Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.
Microscope time | Arrival time (at latest!) | Team colour |
2:05-2:30 | 1:05 | Yellow |
2:30-2:55 | 1:20 | Red |
2:55-3:20 | 1:45 | Blue
mesenchymal stem cells grown in low viscosity alginate with various conditions |
3:20-3:45 | 2:10 | Purple
mesenchymal stem cells grown in low viscosity alginate with various conditions |
3:45-4:10 | 2:35 | Pink
Testing chondrocytes with and without the presence of ascorbate (a growth factor). Beads a made from low viscosity alginate. |
4:10-4:35 | 3:00 | Orange |
4:35-5:00 | 3:25 | Green |
W/F schedule
Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.
Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.
Microscope time | Arrival time (at latest!) | Team colour | |||
2:05-2:30 | 1:05 | Purple | We are testing chondrocytes in 2% low viscosity alginate with different CaCl2 concentrations. | ||
2:30-2:55 | 1:20 | Pink | We tested a cell density of around 1e7 cells in 1.5% low viscosity and 2e6 in 1.5% medium viscosity. | ||
2:55-3:20 | 1:45 | Blue | We tested the impact of different alginate viscosities on chondrocyte differentiation. Cell density was 1 million cells/mL in 1.5% low and 1.5% medium viscosity alginate. | ||
3:20-3:45 | 2:10 | Green | We wanted to see if seeding a stem cell culture with chondrocytes could increase the rate of differentiation. Cell density was 3.6 million cells/mL in 1.5% low viscosity alginate. | ||
3:45-4:10 | 2:35 | Orange | We were testing the influence of BMP-6 upon the differentiation of mesenchymal stem cells into chondrocytes. Cells were cultured in 1% medium-viscosity alginate. | ||
4:10-4:35 | 3:00 | Yellow | We tested chondrocytes on 1.5% medium-viscosity alginate prepared in regular control conditions, or with addition of 800 uM glycine, a major component of collagen. | ||
4:35-5:00 | 3:25 | Red | We tested chondrocytes on 1% medium-viscosity alginate prepared in either 20mM or 200mM CaCl2 by Live/Dead Fluorescence Assay. |