Talk:20.109(S11):Testing cell viability (Day3): Difference between revisions

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mesenchymal stem cells grown in low viscosity alginate with various conditions
mesenchymal stem cells grown in low viscosity alginate with various conditions
[[Image:0.05HAnocollagen.JPG|thumb|center|0.05mg/mL HA and no collagen added]]
[[Image:0.05HAnocollagen.JPG|thumb|center|0.05mg/mL HA and no collagen added]]
[[Image:0.05HAw/collagen.JPG|thumb|center|0.05mg/mL HA and collagen II added]]
[[Image:Collagen.JPG|thumb|center|0.05mg/mL HA and collagen II added]]
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|3:45-4:10
|3:45-4:10

Revision as of 13:32, 21 April 2011

T/R schedule

Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.

Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.

Microscope time Arrival time (at latest!) Team colour
2:05-2:30 1:05 Yellow
2:30-2:55 1:20 Red
2:55-3:20 1:45 Blue

mesenchymal stem cells grown in low viscosity alginate with various conditions

0.5mg/mL HA and no collagen added. Image was taken with the bead core up.
0.5mg/mL HA and approx. 0.1% collagen added. Image was taken with the bead core down. Bead was squished by the coverslip, resulting in lower density of cells in the field of view.
3:20-3:45 2:10 Purple

mesenchymal stem cells grown in low viscosity alginate with various conditions

0.05mg/mL HA and no collagen added
0.05mg/mL HA and collagen II added
3:45-4:10 2:35 Pink
4:10-4:35 3:00 Orange
4:35-5:00 3:25 Green

W/F schedule

Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.

Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.

Microscope time Arrival time (at latest!) Team colour
2:05-2:30 1:05 Purple
2:30-2:55 1:20 Pink
2:55-3:20 1:45 Blue
3:20-3:45 2:10 Green
3:45-4:10 2:35 Orange
4:10-4:35 3:00 Yellow
4:35-5:00 3:25 Red