Talk:20.109(S11):Testing cell viability (Day3)
T/R schedule
Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.
Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.
Microscope time | Arrival time (at latest!) | Team colour |
2:05-2:30 | 1:05 | Yellow |
2:30-2:55 | 1:20 | Red |
2:55-3:20 | 1:45 | Blue
mesenchymal stem cells grown in low viscosity alginate with various conditions |
3:20-3:45 | 2:10 | Purple
mesenchymal stem cells grown in low viscosity alginate with various conditions |
3:45-4:10 | 2:35 | Pink
Testing chondrocytes with and without the presence of ascorbate (a growth factor). Beads a made from low viscosity alginate. |
4:10-4:35 | 3:00 | Orange |
4:35-5:00 | 3:25 | Green |
W/F schedule
Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.
Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.
Microscope time | Arrival time (at latest!) | Team colour | |||
2:05-2:30 | 1:05 | Purple | We are testing chondrocytes in 2% low viscosity alginate with different CaCl2 concentrations. | ||
2:30-2:55 | 1:20 | Pink | We tested a cell density of around 1e7 cells in 1.5% low viscosity and 2e6 in 1.5% medium viscosity. | ||
2:55-3:20 | 1:45 | Blue | |||
3:20-3:45 | 2:10 | Green | |||
3:45-4:10 | 2:35 | Orange | |||
4:10-4:35 | 3:00 | Yellow | |||
4:35-5:00 | 3:25 | Red |