Talk:20.109(S12):Assess protein function (Day7)

Raw Data

Please post your raw calcium titration data below.

Day 7 data file, T/R	Day 7 data file, W/F
Media:S12_M2D7_TR-Red.txt	Media:S12_M2D7_WF-Red.txt
Media:S12_M2D7_TR-Orange.txt	Media:S12_M2D7_WF-Orange.txt
Media:S12_M2D7_TR-Yellow.txt	Media:S12_M2D7_WF-Yellow.txt
Media:S12_M2D7_TR-Green.txt	Media:S12_M2D7_WF-Green.txt
Media:S12_M2D7_TR-Blue.txt	Media:S12_M2D7_WF-Blue.txt
Media:S12_M2D7_TR-Pink.txt	Media:S12_M2D7_WF-Pink.txt
Media:S12_M2D7_TR-Purple.txt	Media:S12_M2D7_WF-Purple.txt

Analysis, T/R

Please be mindful of significant figures when you fill in this table. Do not state far more precise numbers than you can believe.

Group Colour	E67K, T79P, or M124S?	X#Z Mutant	Most believable KD; n for WT	Most believable KD; n for (+)	Most believable KD; n for X#Z mutant	Comments
Orange	T79P	D24H	0.104, 1.26	0.166, 1.30	0.7892, 2.73

Analysis, W/F

Please be mindful of significant figures when you fill in this table. Do not state far more precise numbers than you can believe.

Group Colour	E67K, T79P, or M124S?	X#Z Mutant	Most believable KD; n for WT	Most believable KD; n for (+)	Most believable KD; n for X#Z mutant	Comments
Orange	T79P	V55F	KD 0.1025; n 1.8227 for WT	KD 0.1491; n 1.3982 for T79P	KD 0.1598; n 1.6114 for V55F	The WT and T79P were switched during the experiment. For the above results, the labels were switched back to the original
Red	T79P	T79F	KD 0.1075; n 1.3550 for WT	KD 0.1372; n 1.1433 for T79P	KD 0.1053; n 1.3550 for T79F	As the above group, the labels were switched back to the original between T79P and WT. Also, our yield for WT and T79P was low (may be error prone)