Talk:20.109(S12):Assess protein function (Day7)

Raw Data

Please post your raw calcium titration data below.

Day 7 data file, T/R	Day 7 data file, W/F
Media:S12_M2D7_TR-Red.txt	Media:S12_M2D7_WF-Red.txt
Media:S12_M2D7_TR-Orange.txt	Media:S12_M2D7_WF-Orange.txt
Media:S12_M2D7_TR-Yellow.txt	Media:S12_M2D7_WF-Yellow.txt
Media:S12_M2D7_TR-Green.txt	Media:S12_M2D7_WF-Green.txt
Media:S12_M2D7_TR-Blue.txt	Media:S12_M2D7_WF-Blue.txt
Media:S12_M2D7_TR-Pink.txt	Media:S12_M2D7_WF-Pink.txt
Media:S12_M2D7_TR-Purple.txt	Media:S12_M2D7_WF-Purple.txt

Analysis, T/R

Please be mindful of significant figures when you fill in this table. Do not state far more precise numbers than you can believe.

Group Colour	E67K, T79P, or M124S?	X#Z Mutant	Most believable KD; n for WT	Most believable KD; n for (+)	Most believable KD; n for X#Z mutant	Comments
Orange	T79P	D24H	0.104, 1.26	0.166, 1.30	0.7892, 2.73

Analysis, W/F

Please be mindful of significant figures when you fill in this table. Do not state far more precise numbers than you can believe.

Group Colour	E67K, T79P, or M124S?	X#Z Mutant	Most believable KD; n for WT	Most believable KD; n for (+)	Most believable KD; n for X#Z mutant	Comments
Orange	T79P	V55F	KD 0.1025; n 1.8227 for WT	KD 0.1491; n 1.3982 for T79P	KD 0.1598; n 1.6114 for V55F	The WT and T79P were switched during the experiment. For the above results, the labels were switched back to the original
Red	T79P	T79F	KD 0.1075; n 1.3550 for WT	KD 0.1372; n 1.1433 for T79P	KD 0.1053; n 1.3550 for T79F	As the above group, the labels were switched back to the original between T79P and WT. Also, our yield for WT and T79P was low (may be error prone)
Pink	E67K	D20K	KD 0.1628; n 0.8206 for WT	KD 0.0520; n 0.2212 for E67K	KD 0.000047 for D20K	we used KDs from model 1
Purple	M124S	G23W/G25W	KD 0.1643; n 1.3591 for WT	KD 0.4753; n 2.9004 for M124S	KD 0.1470; n 1.0503 for G23W/G25W	Our yields for WT and G23W/G25W were low. The fluorescence values for G23W/G25W were very low (only between 18 and 24) and may not be significant. For G23W/G25W (contrary to WT and M124S), as [Ca2+] increased, fluorescence increased.