Talk:20.109(S12):Induce protein and evaluate DNA (Day5): Difference between revisions
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Line 25: | Line 25: | ||
|.067 | |.067 | ||
|.024 | |.024 | ||
|Mutant 1 .095 / Mutant 2 .065 | |Mutant 1: .095 / Mutant 2: .065 | ||
|Yes | |Yes | ||
|- | |- |
Revision as of 10:12, 6 April 2012
Induced pellet example
TR O/N Cultures and Gels
Below you should list the OD600 values of the induced cultures (at 1:10 dilutions), as well as your pellet observations. The teaching faculty will fill in this information for the cultures needing to be grown overnight.
Group | OD WT | OD M124S/E67K/T79P | OD Chosen mutant | Pellets yellow? |
---|---|---|---|---|
Red | ||||
Orange | .067 | .024 | Mutant 1: .095 / Mutant 2: .065 | Yes |
Yellow | 0.069 | 0.138 | 0.062 | Yes |
Green | ||||
Blue | ||||
Pink | 0.071 | 0.317 | 0.119 | Y: bright yellow green WT, F65D; dull/pale yellow T79P |
Purple |
Gels
WF O/N Cultures and Gels
Below you should list the OD600 values of the induced cultures (at 1:10 dilutions), as well as your pellet observations. The teaching faculty will fill in this information for the cultures needing to be grown overnight.
Group | OD WT | OD M124S/E67K/T79P | OD Chosen mutant | Pellets yellow? |
---|---|---|---|---|
Red | ||||
Orange | ||||
Yellow | ||||
Green | ||||
Blue | ||||
Pink | ||||
Purple |
Gels