Talk:20.109(S12):Initiate cell culture (Day2): Difference between revisions
| Line 61: | Line 61: | ||
==Designs (T/R)== | ==Designs (T/R)== | ||
Team Orange (with Team Yellow): Papers have shown that TGF Beta 1 is an important growth factor in maintaining a chondrogenic phenotype. Our experiment will examine if there is a dose dependency of TGF Beta 1 on chondrocyte dedifferentiation. We propose testing three different concentrations of TGF Beta 1 | Team Orange (with Team Yellow): Papers have shown that TGF Beta 1 is an important growth factor in maintaining a chondrogenic phenotype. Our experiment will examine if there is a dose dependency of TGF Beta 1 on chondrocyte dedifferentiation. We propose testing three different concentrations of TGF Beta 1 to see at what concentration will they dedifferentiate. | ||
==Designs (W/F)== | ==Designs (W/F)== | ||
Revision as of 12:58, 15 April 2012
Plan for Day 2
Group 1 should arrive by 1:05 pm at the latest and immediately go to the tissue culture room. When everyone has arrived, we will thaw your cells in the water bath. After you have finished your culture preparations (ideally by 3 pm), you can take a 10 minute break to refresh your minds, and then will take a short quiz.
Group 2 should arrive by 2:45 pm at the latest and will begin by taking the quiz. If all goes well, you will begin working in the tissue culture room at 3 pm. If your culture preparations that involve a special physical set-up, you can come earlier and work in the extra tissue culture hood.
Note that you may be asked to switch your group number based on grouping together people working with the same type of cells, asking people who need special reagents/equipment to go in the second group, etc.
Notebooks may be handed in by X dates...
T/R
| Arrival time (at latest!) | 1:05 pm | 2:45 pm |
| Team colour 1 | ||
| Team colour 2 | ||
| Team colour 3 | ||
| Team colour 4 |
W/F
| Arrival time (at latest!) | 1:05 pm | 2:45 pm |
| Team colour 1 | ||
| Team colour 2 | ||
| Team colour 3 | ||
| Team colour 4 |
Designs (T/R)
Team Orange (with Team Yellow): Papers have shown that TGF Beta 1 is an important growth factor in maintaining a chondrogenic phenotype. Our experiment will examine if there is a dose dependency of TGF Beta 1 on chondrocyte dedifferentiation. We propose testing three different concentrations of TGF Beta 1 to see at what concentration will they dedifferentiate.
