Talk:20.109(S12):Initiate cell culture (Day2)

Plan for Day 2

Group 1 should arrive by 1:05 pm at the latest and immediately go to the tissue culture room. When everyone has arrived, we will thaw your cells in the water bath. After you have finished your culture preparations (ideally by 3 pm), you can take a 10 minute break to refresh your minds, and then will take a short quiz.

Group 2 should arrive by 2:45 pm at the latest and will begin by taking the quiz. If all goes well, you will begin working in the tissue culture room at 3 pm. If your culture preparations that involve a special physical set-up, you can come earlier and work in the extra tissue culture hood.

Note that you may be asked to switch your group number based on grouping together people working with the same type of cells, asking people who need special reagents/equipment to go in the second group, etc.

Notebooks may be handed in by the following Tuesday and Wednesday (when we don't have lab), 1 pm outside my office.

T/R

Arrival time (at latest!)	1:05 pm	2:45 pm
Team colour 1		Pink
Team colour 2		Yellow
Team colour 3		Orange (w/Team Yellow)
Team colour 4

W/F

Arrival time (at latest!)	1:05 pm	2:45 pm
Team colour 1	Pink	Blue
Team colour 2	Purple	Yellow
Team colour 3	Orange	Green
Team colour 4	Red

Designs (T/R)

Team Orange (with Team Yellow): Papers have shown that TGF Beta 1 is an important growth factor in maintaining a chondrogenic phenotype. Our experiment will examine if there is a dose dependency of TGF Beta 1 on chondrocyte dedifferentiation. We propose examine how well 0 ng/mL, 2ng/mL and 10ng/mL of TGF B-1 maintains chondrogenic phenotype using 3% low viscosity agarose, and 20x10^6 cells/mL. I think that the chondrocytes with no TGF B-1 will dedifferentiate, because they will be growth factor deficient. (still thinking about 2ng and 10 ng )