Talk:20.109(S12):Purify RNA and run affinity column (Day4): Difference between revisions
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| | |To examine the enrichment at a lower concentration of 8-12. | ||
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Revision as of 12:37, 16 February 2012
Sign-up for column protocols
T/R
You and your partner should propose a protocol in the table below.
If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.
| Protocol# | Group | 8-12 % | Wash # (partner 1) | Wash # (partner 2) | Notes (e.g., short justification for work, whether any other parameter was changed, etc.) | Note changes to plan made on D4 (if any) here |
|---|---|---|---|---|---|---|
| 0 | Sample | 5 | 5 | 34 | Trying maximum wash number done in past. | Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12. |
| 1 | Red | |||||
| 2 | Orange | 1 | 4 | 24 | To examine the enrichment at a lower concentration of 8-12. | |
| 3 | Yellow | |||||
| 4 | Green | |||||
| 5 | Blue | 10 | 10 | 30 | 30 is around max number done in the past. | none yet |
| 6 | Pink | |||||
| 7 | Purple |
W/F
You and your partner should propose a protocol in the table below.
If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.
| Protocol# | Group | 8-12 % | Wash # (partner 1) | Wash # (partner 2) | Notes (e.g., short justification for work, whether any other parameter was changed, etc.) | Note changes to plan made on D4 (if any) here |
|---|---|---|---|---|---|---|
| 0 | Sample | 5 | 5 | 34 | Trying maximum wash number done in past. | Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12. |
| 1 | Red | |||||
| 2 | Orange | |||||
| 3 | Yellow | |||||
| 4 | Green | |||||
| 5 | Blue | |||||
| 6 | Pink | |||||
| 7 | Purple |
