Talk:20.109(S12):Testing cell viability (Day3): Difference between revisions

From OpenWetWare
Jump to navigationJump to search
 
(37 intermediate revisions by 15 users not shown)
Line 28: Line 28:
|3:45-4:10
|3:45-4:10
|2:35
|2:35
|
|Pink
|-
|-
|4:10-4:35
|4:10-4:35
Line 36: Line 36:
|4:35-5:00
|4:35-5:00
|3:25
|3:25
|
|orange
|-
|-
|}
|}
Line 82: Line 82:


==T/R images==
==T/R images==
[[Image:Green TR pic1.jpg|thumb|left|400px|M3D3 Green_TR_1, T/R]]
[[Image:Green TR 2.JPG|thumb|center|400px|M3D3 Green_TR_2, T/R]]
[[Image:Green TR 3.JPG|thumb|left|400px|M3D3 Green_TR_3, T/R]]
[[Image:DSCN2024.JPG|thumb|left|400px|TR_BLUE_CELL_1_CORE_UP, T/R]]
[[Image:DSCN2026.JPG|thumb|center|400px|TR_BLUE_CELL_2_CORE_DOWN, T/R]]
[[Image:DSCN2027.JPG|thumb|left|400px|TR_BLUE_CELL_3_CORE_DOWN, T/R]]
[[Image:DSCN2028.JPG|thumb|center|400px|TR_BLUE_CELL_4_CORE_UP, T/R]]
[[Image:DSCN2002.JPG|thumb|left|400px|TR_Yellow_1_noTGFB, T/R]]
[[Image:DSCN2004.JPG|thumb|center|400px|TR_Yellow_2_noTGFB, T/R]]
[[Image:DSCN2005.JPG|thumb|left|400px|TR_Yellow_3_noTGFB, T/R]]
[[Image:DSCN2006.JPG|thumb|center|400px|TR_Yellow_1_2TGFB, T/R]]
[[Image:DSCN2007.JPG|thumb|left|400px|TR_Yellow_2_2TGFB, T/R]]
[[Image:DSCN2010.JPG|thumb|center|400px|TR_Yellow_3_2TGFB, T/R]]
[[Image:DSCN2058.JPG‎|thumb|left|400px|TR_Orange_1_10 ug/mL TGFB Core, T/R]]
[[Image:DSCN2057.JPG‎|thumb|center|400px|TR_Orange_2_10 ug/mL TGFB Surface, T/R]]
[[Image:DSCN2056.JPG‎|thumb|left|400px|TR_Orange_3_10 ug/mL TGFB Surface, T/R]]
[[Image:DSCN2055.JPG‎|thumb|center|400px|TR_Orange_4_10 ug/mL TGFB Core, T/R]]
[[Image:Red TR FBS Surface.JPG|thumb|left|400px|Red FBS Core]]
[[Image:Red TR NBS.JPG|thumb|left|400px|Red NBS]]
[[Image:TR Pink +HEPES Surface.JPG|thumb|left|400px|TR_Pink_+HEPES_Surface]]
[[Image:TR Pink -HEPES Surface.JPG|thumb|left|400px|TR_Pink_-HEPES_Surface]]
[[Image:TRPurple10.JPG|thumb|left|400px|TR_Purple_1_10TGFB, T/R]]
[[Image:TRPurple10 2.JPG|thumb|center|400px|TR_Purple_2_10TGFB, T/R]]
[[Image:TRPurple10 3.JPG|thumb|left|400px|TR_Purple_3_10TGFB, T/R]]
[[Image:TRPurple20 1.JPG|thumb|center|400px|TR_Purple_1_20TGFB, T/R]]
[[Image:TRPurple20 2.JPG|thumb|left|400px|TR_Purple_2_20TGFB, T/R]]
[[Image:TRPurple20 3.JPG|thumb|center|400px|TR_Purple_3_20TGFB, T/R]]
[[Image:DSCN2116.JPG|thumb|left|400px|WF_Red_TGFB_1, W/F]]
[[Image:DSCN2117.JPG|thumb|center|400px|WF_Red_TGFB_2, W/F]]
[[Image:DSCN2119.JPG|thumb|left|400px|WF_Red_TGFB_3, W/F]]
[[Image:DSCN2123.JPG|thumb|center|400px|WF_Red_BMP2_1, W/F]]
[[Image:DSCN2124.JPG|thumb|left|400px|WF_Red_BMP2_2, W/F]]
[[Image:DSCN2125.JPG|thumb|center|400px|WF_Red_BMP2_3, W/F]]
[[Image:DSCN2141.JPG|thumb|left|400px|WF_Yellow_Insulin_1, W/F]]
[[Image:DSCN2144.JPG|thumb|center|400px|WF_Yellow_Insulin_2, W/F]]
[[Image:DSCN2145.JPG|thumb|left|400px|WF_Yellow_Insulin_3, W/F]]
[[Image:DSCN2155.JPG|thumb|center|400px|WF_Yellow_noInsulin_1, W/F]]
[[Image:DSCN2157.JPG|thumb|left|400px|WF_Yellow_noInsulin_2, W/F]]
[[Image:DSCN2160.JPG|thumb|center|400px|WF_Yellow_noInsulin_3, W/F]]
<br style="clear:both;"/>
==W/F Images==
[[Image:DSCN2078.jpg|thumb|left|400px|WF_Pink_2%FBS+IDT_First Image]]
[[Image:DSCN2079.jpg|thumb|left|400px|WF_Pink_2%FBS+IDT_Second Image]]
[[Image:DSCN2080.jpg|thumb|left|400px|WF_Pink_2%FBS+IDT_Third Image]]
[[Image:DSCN2081.jpg|thumb|left|400px|WF_Pink_10%FBS_First Image]]
[[Image:DSCN2082.jpg|thumb|left|400px|WF_Pink_10%FBS_Second Image]]
[[Image:DSCN2083.jpg|thumb|left|400px|WF_Pink_10%FBS_Third Image]]
[[Image:DSCN2066.JPG|thumb|left|400px|2.5%_Alginate_core_down, WF Purple]]
[[Image:DSCN2071.JPG|thumb|left|400px|1%_Alginate_core_down, WF Purple]]
[[Image:DSCN2129.JPG|thumb|left|400px|Stem Cells:Collagen TGFB Core, WF Orange]
[[Image:DSCN2134.JPG|thumb|left|400px|Stem Cells:Collagen TGFB Border, WF Orange]
[[Image:DSCN2138.JPG|thumb|left|400px|Stem Cells:Collagen BMP Core, WF Orange]
[[Image:DSCN2109.jpg|thumb|left|400px|No Transferrin, WF Green]]
[[Image:DSCN2115.jpg|thumb|left|400px| Transferrin, WF Green]]


==W/F images==
[[Image:DSCN2084.JPG|thumb|left|400px| With Insulin 1, WF Blue]]
[[Image:DSCN2089.JPG|thumb|left|400px| With Insulin 2, WF Blue]]
[[Image:DSCN2090.JPG|thumb|left|400px| With Insulin 3, WF Blue]]
[[Image:DSCN2097.JPG|thumb|left|400px| No Insulin 1 - core up, WF Blue]]
[[Image:DSCN2099.JPG|thumb|left|400px| No Insulin 2 - core down, WF Blue]]
[[Image:DSCN2103.JPG|thumb|left|400px| No Insulin 3, WF Blue]]

Latest revision as of 10:16, 1 May 2012

T/R schedule

Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.

Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.

Microscope time Arrival time (at latest!) Team color
2:05-2:30 1:05 Green
2:30-2:55 1:20 Yellow
2:55-3:20 1:45 Red
3:20-3:45 2:10 Blue
3:45-4:10 2:35 Pink
4:10-4:35 3:00 Purple
4:35-5:00 3:25 orange

W/F schedule

Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.

Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.

Microscope time Arrival time (at latest!) Team Color
2:05-2:30 1:05 Purple
2:30-2:55 1:20 Pink
2:55-3:20 1:45 Blue
3:20-3:45 2:10 Green
3:45-4:10 2:35 Red
4:10-4:35 3:00 Orange
4:35-5:00 3:25 Yellow

T/R images

M3D3 Green_TR_1, T/R
M3D3 Green_TR_2, T/R
M3D3 Green_TR_3, T/R
TR_BLUE_CELL_1_CORE_UP, T/R
TR_BLUE_CELL_2_CORE_DOWN, T/R
TR_BLUE_CELL_3_CORE_DOWN, T/R
TR_BLUE_CELL_4_CORE_UP, T/R
TR_Yellow_1_noTGFB, T/R
TR_Yellow_2_noTGFB, T/R
TR_Yellow_3_noTGFB, T/R
TR_Yellow_1_2TGFB, T/R
TR_Yellow_2_2TGFB, T/R
TR_Yellow_3_2TGFB, T/R
TR_Orange_1_10 ug/mL TGFB Core, T/R
TR_Orange_2_10 ug/mL TGFB Surface, T/R
TR_Orange_3_10 ug/mL TGFB Surface, T/R
TR_Orange_4_10 ug/mL TGFB Core, T/R
Red FBS Core
Red NBS
TR_Pink_+HEPES_Surface
TR_Pink_-HEPES_Surface


TR_Purple_1_10TGFB, T/R
TR_Purple_2_10TGFB, T/R
TR_Purple_3_10TGFB, T/R
TR_Purple_1_20TGFB, T/R
TR_Purple_2_20TGFB, T/R
TR_Purple_3_20TGFB, T/R
WF_Red_TGFB_1, W/F
WF_Red_TGFB_2, W/F
WF_Red_TGFB_3, W/F
WF_Red_BMP2_1, W/F
WF_Red_BMP2_2, W/F
WF_Red_BMP2_3, W/F
WF_Yellow_Insulin_1, W/F
WF_Yellow_Insulin_2, W/F
WF_Yellow_Insulin_3, W/F
WF_Yellow_noInsulin_1, W/F
WF_Yellow_noInsulin_2, W/F
WF_Yellow_noInsulin_3, W/F


W/F Images

WF_Pink_2%FBS+IDT_First Image
WF_Pink_2%FBS+IDT_Second Image
WF_Pink_2%FBS+IDT_Third Image
WF_Pink_10%FBS_First Image
WF_Pink_10%FBS_Second Image
WF_Pink_10%FBS_Third Image


2.5%_Alginate_core_down, WF Purple
1%_Alginate_core_down, WF Purple

[[Image:DSCN2129.JPG|thumb|left|400px|Stem Cells:Collagen TGFB Core, WF Orange] [[Image:DSCN2134.JPG|thumb|left|400px|Stem Cells:Collagen TGFB Border, WF Orange] [[Image:DSCN2138.JPG|thumb|left|400px|Stem Cells:Collagen BMP Core, WF Orange]

No Transferrin, WF Green
Transferrin, WF Green
With Insulin 1, WF Blue
With Insulin 2, WF Blue
With Insulin 3, WF Blue
No Insulin 1 - core up, WF Blue
No Insulin 2 - core down, WF Blue
No Insulin 3, WF Blue