Talk:20.109(S12):Testing cell viability (Day3)

T/R schedule

Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.

Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.

Microscope time	Arrival time (at latest!)	Team color
2:05-2:30	1:05	Green
2:30-2:55	1:20	Yellow
2:55-3:20	1:45	Red
3:20-3:45	2:10	Blue
3:45-4:10	2:35	Pink
4:10-4:35	3:00	Purple
4:35-5:00	3:25	orange

W/F schedule

Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.

Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.

Microscope time	Arrival time (at latest!)	Team Color
2:05-2:30	1:05	Purple
2:30-2:55	1:20	Pink
2:55-3:20	1:45	Blue
3:20-3:45	2:10	Green
3:45-4:10	2:35	Red
4:10-4:35	3:00	Orange
4:35-5:00	3:25	Yellow

T/R images

M3D3 Green_TR_1, T/R

M3D3 Green_TR_2, T/R

M3D3 Green_TR_3, T/R

TR_BLUE_CELL_1_CORE_UP, T/R

TR_BLUE_CELL_2_CORE_DOWN, T/R

TR_BLUE_CELL_3_CORE_DOWN, T/R

TR_BLUE_CELL_4_CORE_UP, T/R

TR_Yellow_1_noTGFB, T/R

TR_Yellow_2_noTGFB, T/R

TR_Yellow_3_noTGFB, T/R

TR_Yellow_1_2TGFB, T/R

TR_Yellow_2_2TGFB, T/R

TR_Yellow_3_2TGFB, T/R

TR_Orange_1_10 ug/mL TGFB Core, T/R

TR_Orange_2_10 ug/mL TGFB Surface, T/R

TR_Orange_3_10 ug/mL TGFB Surface, T/R

TR_Orange_4_10 ug/mL TGFB Core, T/R

Red FBS Core

Red NBS

TR_Pink_+HEPES_Surface

TR_Pink_-HEPES_Surface

TR_Purple_1_10TGFB, T/R

TR_Purple_2_10TGFB, T/R

TR_Purple_3_10TGFB, T/R

TR_Purple_1_20TGFB, T/R

TR_Purple_2_20TGFB, T/R

TR_Purple_3_20TGFB, T/R

WF_Red_TGFB_1, W/F

WF_Red_TGFB_2, W/F

WF_Red_TGFB_3, W/F

WF_Red_BMP2_1, W/F

WF_Red_BMP2_2, W/F

WF_Red_BMP2_3, W/F

WF_Yellow_Insulin_1, W/F

WF_Yellow_Insulin_2, W/F

WF_Yellow_Insulin_3, W/F

WF_Yellow_noInsulin_1, W/F

WF_Yellow_noInsulin_2, W/F

WF_Yellow_noInsulin_3, W/F

W/F Images

WF_Pink_2%FBS+IDT_First Image

WF_Pink_2%FBS+IDT_Second Image

WF_Pink_2%FBS+IDT_Third Image

WF_Pink_10%FBS_First Image

WF_Pink_10%FBS_Second Image

WF_Pink_10%FBS_Third Image

2.5%_Alginate_core_down, WF Purple

1%_Alginate_core_down, WF Purple

[[Image:DSCN2129.JPG|thumb|left|400px|Stem Cells:Collagen TGFB Core, WF Orange] [[Image:DSCN2134.JPG|thumb|left|400px|Stem Cells:Collagen TGFB Border, WF Orange] [[Image:DSCN2138.JPG|thumb|left|400px|Stem Cells:Collagen BMP Core, WF Orange]

No Transferrin, WF Green

Transferrin, WF Green

With Insulin 1, WF Blue

With Insulin 2, WF Blue

With Insulin 3, WF Blue

No Insulin 1 - core up, WF Blue

No Insulin 2 - core down, WF Blue

No Insulin 3, WF Blue