Talk:20.109(S12):Testing cell viability (Day3): Difference between revisions

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==T/R images==
==T/R images==
[[Image:Green TR pic1.jpg|thumb|left|400px|M3D3 Green_TR_1, T/R]]
[[Image:Green TR pic1.jpg|thumb|left|400px|M3D3 Green_TR_1, T/R]]
[[Image:Green TR 2.JPG|thumb|left|400px|M3D3 Green_TR_2, T/R]]
[[Image:Green TR 2.JPG|thumb|center|400px|M3D3 Green_TR_2, T/R]]
[[Image:Green TR 3.JPG|thumb|left|400px|M3D3 Green_TR_3, T/R]]
[[Image:Green TR 3.JPG|thumb|left|400px|M3D3 Green_TR_3, T/R]]


[[Image:DSCN2024.JPG|thumb|left|400px|TR_BLUE_CELL_1_CORE_UP, T/R]]
[[Image:DSCN2024.JPG|thumb|left|400px|TR_BLUE_CELL_1_CORE_UP, T/R]]
[[Image:DSCN2026.JPG|thumb|left|400px|TR_BLUE_CELL_2_CORE_DOWN, T/R]]
[[Image:DSCN2026.JPG|thumb|center|400px|TR_BLUE_CELL_2_CORE_DOWN, T/R]]
[[Image:DSCN2027.JPG|thumb|left|400px|TR_BLUE_CELL_3_CORE_DOWN, T/R]]
[[Image:DSCN2027.JPG|thumb|left|400px|TR_BLUE_CELL_3_CORE_DOWN, T/R]]
[[Image:DSCN2028.JPG|thumb|left|400px|TR_BLUE_CELL_4_CORE_UP, T/R]]
[[Image:DSCN2028.JPG|thumb|center|400px|TR_BLUE_CELL_4_CORE_UP, T/R]]


[[Image:DSCN2002.JPG|thumb|left|400px|TR_Yellow_1_noTGFB, T/R]]
[[Image:DSCN2002.JPG|thumb|left|400px|TR_Yellow_1_noTGFB, T/R]]
[[Image:DSCN2004.JPG|thumb|left|400px|TR_Yellow_2_noTGFB, T/R]]
[[Image:DSCN2004.JPG|thumb|center|400px|TR_Yellow_2_noTGFB, T/R]]
[[Image:DSCN2005.JPG|thumb|left|400px|TR_Yellow_3_noTGFB, T/R]]
[[Image:DSCN2005.JPG|thumb|left|400px|TR_Yellow_3_noTGFB, T/R]]
[[Image:DSCN2006.JPG|thumb|left|400px|TR_Yellow_1_2TGFB, T/R]]
[[Image:DSCN2006.JPG|thumb|center|400px|TR_Yellow_1_2TGFB, T/R]]
[[Image:DSCN2007.JPG|thumb|left|400px|TR_Yellow_2_2TGFB, T/R]]
[[Image:DSCN2007.JPG|thumb|left|400px|TR_Yellow_2_2TGFB, T/R]]
[[Image:DSCN2010.JPG|thumb|left|400px|TR_Yellow_3_2TGFB, T/R]]
[[Image:DSCN2010.JPG|thumb|center|400px|TR_Yellow_3_2TGFB, T/R]]


[[Image:DSCN2058.JPG‎|thumb|left|400px|TR_Orange_1_10TGFB, T/R]]
[[Image:DSCN2058.JPG‎|thumb|left|400px|TR_Orange_1_10TGFB, T/R]]
[[Image:DSCN2057.JPG‎|thumb|left|400px|TR_Orange_2_10TGFB, T/R]]
[[Image:DSCN2057.JPG‎|thumb|center|400px|TR_Orange_2_10TGFB, T/R]]
[[Image:DSCN2056.JPG‎|thumb|left|400px|TR_Orange_3_10TGFB, T/R]]
[[Image:DSCN2056.JPG‎|thumb|left|400px|TR_Orange_3_10TGFB, T/R]]
[[Image:DSCN2055.JPG‎|thumb|left|400px|TR_Orange_4_10TGFB, T/R]]
[[Image:DSCN2055.JPG‎|thumb|center|400px|TR_Orange_4_10TGFB, T/R]]


[[Image:Red TR FBS Surface.JPG|thumb|left|400px|Red FBS Core]]
[[Image:Red TR FBS Surface.JPG|thumb|left|400px|Red FBS Core]]
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[[Image:TRPurple10.JPG|thumb|left|400px|TR_Purple_1_10TGFB, T/R]]
[[Image:TRPurple10.JPG|thumb|left|400px|TR_Purple_1_10TGFB, T/R]]
[[Image:TRPurple10 2.JPG|thumb|left|400px|TR_Purple_2_10TGFB, T/R]]
[[Image:TRPurple10 2.JPG|thumb|center|400px|TR_Purple_2_10TGFB, T/R]]
[[Image:TRPurple10 3.JPG|thumb|left|400px|TR_Purple_3_10TGFB, T/R]]
[[Image:TRPurple10 3.JPG|thumb|left|400px|TR_Purple_3_10TGFB, T/R]]
[[Image:TRPurple20 1.JPG|thumb|left|400px|TR_Purple_1_20TGFB, T/R]]
[[Image:TRPurple20 1.JPG|thumb|center|400px|TR_Purple_1_20TGFB, T/R]]
[[Image:TRPurple20 2.JPG|thumb|left|400px|TR_Purple_2_20TGFB, T/R]]
[[Image:TRPurple20 2.JPG|thumb|left|400px|TR_Purple_2_20TGFB, T/R]]
[[Image:TRPurple20 3.JPG|thumb|left|400px|TR_Purple_3_20TGFB, T/R]]
[[Image:TRPurple20 3.JPG|thumb|center|400px|TR_Purple_3_20TGFB, T/R]]


[[Image:DSCN2116.JPG|thumb|left|400px|WF_Red_TGFB_1, W/F]]
[[Image:DSCN2116.JPG|thumb|left|400px|WF_Red_TGFB_1, W/F]]
[[Image:DSCN2117.JPG|thumb|left|400px|WF_Red_TGFB_2, W/F]]
[[Image:DSCN2117.JPG|thumb|center|400px|WF_Red_TGFB_2, W/F]]
[[Image:DSCN2119.JPG|thumb|left|400px|WF_Red_TGFB_3, W/F]]
[[Image:DSCN2119.JPG|thumb|left|400px|WF_Red_TGFB_3, W/F]]
[[Image:DSCN2123.JPG|thumb|left|400px|WF_Red_BMP2_1, W/F]]
[[Image:DSCN2123.JPG|thumb|center|400px|WF_Red_BMP2_1, W/F]]
[[Image:DSCN2124.JPG|thumb|left|400px|WF_Red_BMP2_2, W/F]]
[[Image:DSCN2124.JPG|thumb|left|400px|WF_Red_BMP2_2, W/F]]
[[Image:DSCN2125.JPG|thumb|left|400px|WF_Red_BMP2_3, W/F]]
[[Image:DSCN2125.JPG|thumb|center|400px|WF_Red_BMP2_3, W/F]]


[[Image:DSCN2141.JPG|thumb|left|400px|WF_Yellow_Insulin_1, W/F]]
[[Image:DSCN2141.JPG|thumb|left|400px|WF_Yellow_Insulin_1, W/F]]
[[Image:DSCN2144.JPG|thumb|left|400px|WF_Yellow_Insulin_2, W/F]]
[[Image:DSCN2144.JPG|thumb|center|400px|WF_Yellow_Insulin_2, W/F]]
[[Image:DSCN2145.JPG|thumb|left|400px|WF_Yellow_Insulin_3, W/F]]
[[Image:DSCN2145.JPG|thumb|left|400px|WF_Yellow_Insulin_3, W/F]]
[[Image:DSCN2155.JPG|thumb|left|400px|WF_Yellow_noInsulin_1, W/F]]
[[Image:DSCN2155.JPG|thumb|center|400px|WF_Yellow_noInsulin_1, W/F]]
[[Image:DSCN2157.JPG|thumb|left|400px|WF_Yellow_noInsulin_2, W/F]]
[[Image:DSCN2157.JPG|thumb|left|400px|WF_Yellow_noInsulin_2, W/F]]
[[Image:DSCN2160.JPG|thumb|left|400px|WF_Yellow_noInsulin_3, W/F]]
[[Image:DSCN2160.JPG|thumb|center|400px|WF_Yellow_noInsulin_3, W/F]]


<br style="clear:both;"/>
==W/F Images==
==W/F Images==
[[Image:DSCN2078.jpg|thumb|left|400px|WF_Pink_2%FBS+IDT_First Image]]
[[Image:DSCN2078.jpg|thumb|left|400px|WF_Pink_2%FBS+IDT_First Image]]

Revision as of 06:46, 1 May 2012

T/R schedule

Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.

Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.

Microscope time Arrival time (at latest!) Team color
2:05-2:30 1:05 Green
2:30-2:55 1:20 Yellow
2:55-3:20 1:45 Red
3:20-3:45 2:10 Blue
3:45-4:10 2:35 Pink
4:10-4:35 3:00 Purple
4:35-5:00 3:25 orange

W/F schedule

Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.

Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.

Microscope time Arrival time (at latest!) Team Color
2:05-2:30 1:05 Purple
2:30-2:55 1:20 Pink
2:55-3:20 1:45 Blue
3:20-3:45 2:10 Green
3:45-4:10 2:35 Red
4:10-4:35 3:00 Orange
4:35-5:00 3:25 Yellow

T/R images

M3D3 Green_TR_1, T/R
M3D3 Green_TR_2, T/R
M3D3 Green_TR_3, T/R
TR_BLUE_CELL_1_CORE_UP, T/R
TR_BLUE_CELL_2_CORE_DOWN, T/R
TR_BLUE_CELL_3_CORE_DOWN, T/R
TR_BLUE_CELL_4_CORE_UP, T/R
TR_Yellow_1_noTGFB, T/R
TR_Yellow_2_noTGFB, T/R
TR_Yellow_3_noTGFB, T/R
TR_Yellow_1_2TGFB, T/R
TR_Yellow_2_2TGFB, T/R
TR_Yellow_3_2TGFB, T/R
TR_Orange_1_10TGFB, T/R
TR_Orange_2_10TGFB, T/R
TR_Orange_3_10TGFB, T/R
TR_Orange_4_10TGFB, T/R
Red FBS Core
Red NBS
TR_Pink_+HEPES_Surface
TR_Pink_-HEPES_Surface


TR_Purple_1_10TGFB, T/R
TR_Purple_2_10TGFB, T/R
TR_Purple_3_10TGFB, T/R
TR_Purple_1_20TGFB, T/R
TR_Purple_2_20TGFB, T/R
TR_Purple_3_20TGFB, T/R
WF_Red_TGFB_1, W/F
WF_Red_TGFB_2, W/F
WF_Red_TGFB_3, W/F
WF_Red_BMP2_1, W/F
WF_Red_BMP2_2, W/F
WF_Red_BMP2_3, W/F
WF_Yellow_Insulin_1, W/F
WF_Yellow_Insulin_2, W/F
WF_Yellow_Insulin_3, W/F
WF_Yellow_noInsulin_1, W/F
WF_Yellow_noInsulin_2, W/F
WF_Yellow_noInsulin_3, W/F


W/F Images

WF_Pink_2%FBS+IDT_First Image
WF_Pink_2%FBS+IDT_Second Image
WF_Pink_2%FBS+IDT_Third Image
WF_Pink_10%FBS_First Image
WF_Pink_10%FBS_Second Image
WF_Pink_10%FBS_Third Image


2.5%_Alginate_core_down, WF Purple
1%_Alginate_core_down, WF Purple


No Transferrin, WF Green
Transferrin, WF Green
With Insulin 1, WF Blue
With Insulin 2, WF Blue
With Insulin 3, WF Blue
No Insulin 1 - core up, WF Blue
No Insulin 2 - core down, WF Blue
No Insulin 3, WF Blue