Talk:20.109(S13):Characterize protein expression (Day7): Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 9: | Line 9: | ||
===T/R=== | ===T/R=== | ||
[[Image: | [[Image:S13_M2D7_TR_Red.JPG | right |thumb|250px | T/R Gel, Red group]] | ||
[[Image: | [[Image:S13_M2D7_TR_Orange.JPG | left |thumb|250px | T/R Gel, Orange group]] | ||
[[Image: | [[Image:S13_M2D7_TR_Yellow.JPG | center |thumb|250px | T/R Gel, Yellow group]] | ||
[[Image: | [[Image:S13_M2D7_TR_Green.JPG | right |thumb|250px | T/R Gel, Green group]] | ||
[[Image: | [[Image:S13_M2D7_TR_Blue.JPG | left |thumb|250px | T/R Gel, Blue group]] | ||
[[Image: | [[Image:S13_M2D7_TR_Purple.JPG | center |thumb|250px | T/R Gel, Purple group]] | ||
[[Image: | [[Image:S13_M2D7_TR_Platinum.JPG | left |thumb|250px | T/R Gel, Platinum group]] | ||
<br style="clear:both;"/> | |||
===W/F=== | ===W/F=== |
Revision as of 12:43, 10 April 2013
SDS-PAGE analysis tips
You can look up the expected molecular weight of IPC using the sequence file (from Day 1 or Day 5) and this link or a similar website. Be sure to add ~ 3 KDa for the size of the N-terminus of pRSET (His tag, etc)!
If you see two strong bands on your gel, what do you think the second one is? As a starting hint, look back at the Day 6 protocol and carefully consider what is present in the unpurified protein tubes.
SDS-PAGE images
T/R