Talk:20.109(S13):Characterize protein expression (Day7)

From OpenWetWare
Revision as of 11:27, 11 April 2013 by AgiStachowiak (talk | contribs)
Jump to navigationJump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.

SDS-PAGE analysis tips

You can look up the expected molecular weight of IPC using the sequence file (from Day 1 or Day 5) and this link or a similar website. Be sure to add ~ 3 KDa for the size of the N-terminus of pRSET (His tag, etc)!

If you see two strong bands on your gel, what do you think the second one is? As a starting hint, look back at the Day 6 protocol and carefully consider what is present in the unpurified protein tubes.

What other assays might you consider in relation to SDS-PAGE in order to take a holistic view of your data?

SDS-PAGE images

T/R

T/R Gel, Red group
T/R Gel, Orange group
T/R Gel, Yellow group
T/R Gel, Green group
T/R Gel, Blue group (broke a little, sorry)
T/R Gel, Purple group
T/R Gel, Platinum group


W/F

T/R Gel, Red group
T/R Gel, Orange group
T/R Gel, Yellow group
T/R Gel, Green group
T/R Gel, Blue group
File:S13 M2D7 TR Pink.JPG
T/R Gel, Pink group
T/R Gel, Purple group
T/R Gel, Platinum group


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Talk:20.109(S13):Characterize_protein_expression_(Day7)&oldid=690595"