Talk:20.109(S13):Induce protein and evaluate DNA (Day5)

Induced pellet example

Examples of induced (left) and control (right) cell pellets

TR O/N Cultures and Gels

Below you should list the OD₆₀₀ values of the induced cultures (stock value), as well as your pellet observations. (Is the color dull or bright? More yellow or more green?) The teaching faculty will fill in this information for the cultures needing to be grown overnight.

Group	OD WT	OD D24H/E67K/T79P/M124S	OD Chosen mutant	Pellets yellow?
Example	0.95	1.2 (D24H)	0.82 (G20P)	Y, bright ylw-grn
Red	0.22	0.51 (D24H)	0.55	grn, grn, ylw (+hint of grn)
Orange	0.36	0.36 (D24H)	0.38	grn, grn, ylw (+hint of grn)
Yellow	0.39	2.12 (T79P)	3.36 (K77E2--not the chosen mutant) extra is 0.17	bright yellow; ylw (+hint of grn)
Green	0.34	1.59 (T79P)	1.16 (M71R)	Bright Yellow
Blue	0.035	0.036 (M124S)	0.088 (M71S)	Y, bright ylw
Purple	0.30	0.58 (M124S)	0.32	ylw-grn, ylw-grn, grn
Platinum	0.52	0.75 (M124S)	0.38	ylw-grn, ylw-grn, grn

Gels

T/R Red

T/R Orange

T/R Yellow

T/R Green

T/R Blue

T/R Purple

T/R Platinum

WF O/N Cultures and Gels

Below you should list the OD₆₀₀ values of the induced cultures (stock value), as well as your pellet observations. (Is the color dull or bright? More yellow or more green?) The teaching faculty will fill in this information for the cultures needing to be grown overnight.

Group	OD WT	OD D24H/E67K/T79P/M124S	OD mutant option 1	OD mutant option 2	Pellets yellow?
Example	0.95	1.2 (D24H)	0.82 (G20P)	N/A	Y, bright ylw-grn
Red
Orange
Yellow
Green
Blue
Pink
Purple
Platinum

Gels

File:S13 M2D5 WF gel-Red.jpg

W/F Red

W/F Orange

W/F Yellow

W/F Green

W/F Blue

W/F Pink

W/F Purple

W/F Platinum