Talk:20.109(S13):Transcript-level analysis (Day6): Difference between revisions
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[[Image:S13_M3D5_TR_noiseband.jpg|thumb|400px|center|T/R section, Log-scale amplification curves with noise threshold]] | [[Image:S13_M3D5_TR_noiseband.jpg|thumb|400px|center|T/R section, Log-scale amplification curves with noise threshold]] | ||
[[Image:S13_M3D5_TR_18S-melt.jpg|thumb|300px|right|T/R section, Melt curves for | [[Image:S13_M3D5_TR_18S-melt.jpg|thumb|300px|right|T/R section, Melt curves for 18S samples]] | ||
[[Image:S13_M3D5_TR_CNII-melt.jpg|thumb|300px|left|T/R section, Melt curves for CN II samples]] | [[Image:S13_M3D5_TR_CNII-melt.jpg|thumb|300px|left|T/R section, Melt curves for CN II samples]] | ||
[[Image:S13_M3D5_TR_CNI-melt.jpg|thumb|300px|center|T/R section, Melt curves for CN II samples]] | [[Image:S13_M3D5_TR_CNI-melt.jpg|thumb|300px|center|T/R section, Melt curves for CN II samples]] | ||
[[Image:S13_M3D5_TR_18S-noiseband.jpg|thumb|300px|right|T/R section, Amp curves for 18S samples]] | |||
[[Image:S13_M3D5_TR_CNII-noiseband.jpg|thumb|300px|left|T/R section, Amp curves for CN II samples]] | |||
[[Image:S13_M3D5_TR_CNI-noiseband.jpg|thumb|300px|center|T/R section, Amp curves for CN II samples]] | |||
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Revision as of 12:24, 6 May 2013
qPCR data
T/R
The Excel file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. (T/R Purple, because your samples ran separately, there were not enough of them to make a per RNA threshold reasonable.)
W/F
The Excel file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set.
qPCR section-wide curves
T/R
W/F
Cross-group meetings
T/R Scheme
Red 1/Yellow 1: Marisol/Dominique
Red 2/Blue 1: Nick/Justin
Orange 1/Platinum 2: Daphne/Katie
Platinum 1/Blue 2: Emily/Noa
Yellow 2/Green 2: Ebaa/Jesse
Green 1/Purple 1:Nikki/Sarah
Orange 2/Purple 2: Stas/Mitali
