Talk:20.109(S14):Cell preparation for DNA repair assays (Day5): Difference between revisions
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Concentrations of TA | ==Concentrations of Student Prepped DNA== | ||
Note: All TA prepped DNA will be at 50 ng/μL. | |||
{| border="1" | {| border="1" | ||
! Cut topology | ! Cut topology | ||
! Group 1 | ! Group 1 | ||
! Concentration | ! Concentration (ng/uL) | ||
! Group 2 | ! Group 2 | ||
! Concentration | ! Concentration (ng/uL) | ||
! (Group 3) | ! (Group 3) | ||
! (Concentration) | ! (Concentration (ng/uL)) | ||
|- | |- | ||
| Sticky ends, 5' overhangs | | Sticky ends, 5' overhangs | ||
| Orange Team (TR) | | Orange Team (TR) | ||
| | | 27.0 | ||
| Red Team (WF) | | Red Team (WF) | ||
| 18.7 | |||
| | | | ||
| | | | ||
| Line 19: | Line 22: | ||
| Sticky ends, 3' overhangs | | Sticky ends, 3' overhangs | ||
|Blue Team (TR) | |Blue Team (TR) | ||
|28.5 | |||
|Purple Team (WF) | |Purple Team (WF) | ||
|11.0 | |||
| | |||
| | | | ||
|- | |- | ||
| Blunt | | Blunt | ||
|Yellow Team (TR) | |Yellow Team (TR) | ||
|21.4 | |||
|Yellow Team (WF) | |Yellow Team (WF) | ||
|27.3 | |||
|Blue Team (WF) | |Blue Team (WF) | ||
|34.0 | |||
|- | |- | ||
| Hybrid: 5' sticky upstream, blunt downstream | | Hybrid: 5' sticky upstream, blunt downstream | ||
|Pink Team (TR) | |Pink Team (TR) | ||
|34.9 | |||
|Green Team (WF) | |Green Team (WF) | ||
|34.6 | |||
| | |||
| | | | ||
|- | |- | ||
| Hybrid: 3' sticky upstream, blunt downstream | | Hybrid: 3' sticky upstream, blunt downstream | ||
|Red Team (TR) | |Red Team (TR) | ||
|21.8 | |||
|Pink Team (WF) | |Pink Team (WF) | ||
|51.8 | |||
| | |||
| | | | ||
|- | |- | ||
| Sticky ends with sequence mismatch | | Sticky ends with sequence mismatch | ||
|Green Team (TR) | |Green Team (TR) | ||
|29.7 | |||
|White Team (WF) | |White Team (WF) | ||
|18.1 | |||
| | |||
| | | | ||
|- | |- | ||
| Sticky ends with sequence and topology mismatch | | Sticky ends with sequence and topology mismatch | ||
|BA Orange Team (WF) | |BA Orange Team (WF) | ||
|12.6 | |||
|not so BA Silver Team (WF) | |not so BA Silver Team (WF) | ||
|33.7 | |||
| | |||
| | | | ||
|- | |- | ||
|} | |} | ||
Latest revision as of 13:00, 28 March 2014
Concentrations of Student Prepped DNA
Note: All TA prepped DNA will be at 50 ng/μL.
| Cut topology | Group 1 | Concentration (ng/uL) | Group 2 | Concentration (ng/uL) | (Group 3) | (Concentration (ng/uL)) |
|---|---|---|---|---|---|---|
| Sticky ends, 5' overhangs | Orange Team (TR) | 27.0 | Red Team (WF) | 18.7 | ||
| Sticky ends, 3' overhangs | Blue Team (TR) | 28.5 | Purple Team (WF) | 11.0 | ||
| Blunt | Yellow Team (TR) | 21.4 | Yellow Team (WF) | 27.3 | Blue Team (WF) | 34.0 |
| Hybrid: 5' sticky upstream, blunt downstream | Pink Team (TR) | 34.9 | Green Team (WF) | 34.6 | ||
| Hybrid: 3' sticky upstream, blunt downstream | Red Team (TR) | 21.8 | Pink Team (WF) | 51.8 | ||
| Sticky ends with sequence mismatch | Green Team (TR) | 29.7 | White Team (WF) | 18.1 | ||
| Sticky ends with sequence and topology mismatch | BA Orange Team (WF) | 12.6 | not so BA Silver Team (WF) | 33.7 |
