Talk:20.109(S14):Cell preparation for DNA repair assays (Day5): Difference between revisions
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| Line 12: | Line 12: | ||
| Sticky ends, 5' overhangs | | Sticky ends, 5' overhangs | ||
| Orange Team (TR) | | Orange Team (TR) | ||
| | | 65.1 | ||
| Red Team (WF) | | Red Team (WF) | ||
| | | 59.5 | ||
| | | | ||
| | | | ||
| Line 20: | Line 20: | ||
| Sticky ends, 3' overhangs | | Sticky ends, 3' overhangs | ||
|Blue Team (TR) | |Blue Team (TR) | ||
| | |73.9 | ||
|Purple Team (WF) | |Purple Team (WF) | ||
| | |60.6 | ||
| | | | ||
| | | | ||
| Line 28: | Line 28: | ||
| Blunt | | Blunt | ||
|Yellow Team (TR) | |Yellow Team (TR) | ||
| | |67.7 | ||
|Yellow Team (WF) | |Yellow Team (WF) | ||
| | |60.7 | ||
|Blue Team (WF) | |Blue Team (WF) | ||
|60.4 | |||
| | | | ||
|- | |- | ||
| Hybrid: 5' sticky upstream, blunt downstream | | Hybrid: 5' sticky upstream, blunt downstream | ||
|Pink Team (TR) | |Pink Team (TR) | ||
| | |65.9 | ||
|Green Team (WF) | |Green Team (WF) | ||
| | |62.7 | ||
| | | | ||
| | | | ||
| Line 44: | Line 45: | ||
| Hybrid: 3' sticky upstream, blunt downstream | | Hybrid: 3' sticky upstream, blunt downstream | ||
|Red Team (TR) | |Red Team (TR) | ||
| | |65.5 | ||
|Pink Team (WF) | |Pink Team (WF) | ||
| | |74.3 | ||
| | | | ||
| | | | ||
| Line 52: | Line 53: | ||
| Sticky ends with sequence mismatch | | Sticky ends with sequence mismatch | ||
|Green Team (TR) | |Green Team (TR) | ||
| | |65.6 | ||
|White Team (WF) | |White Team (WF) | ||
| | |62.9 | ||
| | | | ||
| | | | ||
| Line 60: | Line 61: | ||
| Sticky ends with sequence and topology mismatch | | Sticky ends with sequence and topology mismatch | ||
|BA Orange Team (WF) | |BA Orange Team (WF) | ||
| | |62.8 | ||
|not so BA Silver Team (WF) | |not so BA Silver Team (WF) | ||
| | |53.0 | ||
| | | | ||
| | | | ||
|- | |- | ||
|} | |} | ||
Revision as of 11:45, 28 March 2014
Concentrations of TA Prepped DNA
| Cut topology | Group 1 | Concentration | Group 2 | Concentration | (Group 3) | (Concentration) | |
|---|---|---|---|---|---|---|---|
| Sticky ends, 5' overhangs | Orange Team (TR) | 65.1 | Red Team (WF) | 59.5 | |||
| Sticky ends, 3' overhangs | Blue Team (TR) | 73.9 | Purple Team (WF) | 60.6 | |||
| Blunt | Yellow Team (TR) | 67.7 | Yellow Team (WF) | 60.7 | Blue Team (WF) | 60.4 | |
| Hybrid: 5' sticky upstream, blunt downstream | Pink Team (TR) | 65.9 | Green Team (WF) | 62.7 | |||
| Hybrid: 3' sticky upstream, blunt downstream | Red Team (TR) | 65.5 | Pink Team (WF) | 74.3 | |||
| Sticky ends with sequence mismatch | Green Team (TR) | 65.6 | White Team (WF) | 62.9 | |||
| Sticky ends with sequence and topology mismatch | BA Orange Team (WF) | 62.8 | not so BA Silver Team (WF) | 53.0 |
