Talk:20.109(S14):Cell preparation for DNA repair assays (Day5): Difference between revisions
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| Line 8: | Line 8: | ||
! Concentration | ! Concentration | ||
! (Group 3) | ! (Group 3) | ||
! Concentration | ! (Concentration) | ||
|- | |- | ||
| Sticky ends, 5' overhangs | | Sticky ends, 5' overhangs | ||
| Orange Team (TR) | | Orange Team (TR) | ||
| | |||
| Red Team (WF) | | Red Team (WF) | ||
| | |||
| | | | ||
|- | |- | ||
Revision as of 11:20, 28 March 2014
Concentrations of TA Prepped DNA
| Cut topology | Group 1 | Concentration | Group 2 | Concentration | (Group 3) | (Concentration) |
|---|---|---|---|---|---|---|
| Sticky ends, 5' overhangs | Orange Team (TR) | Red Team (WF) | ||||
| Sticky ends, 3' overhangs | Blue Team (TR) | Purple Team (WF) | ||||
| Blunt | Yellow Team (TR) | Yellow Team (WF) | Blue Team (WF) | |||
| Hybrid: 5' sticky upstream, blunt downstream | Pink Team (TR) | Green Team (WF) | ||||
| Hybrid: 3' sticky upstream, blunt downstream | Red Team (TR) | Pink Team (WF) | ||||
| Sticky ends with sequence mismatch | Green Team (TR) | White Team (WF) | ||||
| Sticky ends with sequence and topology mismatch | BA Orange Team (WF) | not so BA Silver Team (WF) |
