Talk:20.109(S14):Diagnostic primer design (Day2)

T/R Section

Sensitivity

Team color	Forward (F) primer	Reverse (R) primer	Target site (e.g. bp 60), F	Target side (e.g., bp 300), R	Is bp # for VC or EH? Which gene?
Red	CAC CAG GTT GAT ACT GCC TGA C	CCT ATC CCG AAC CAA ACC CTG	bp 1	bp 260	EH, SSU rRNA
Green
Pink	ACC AGG TTG ATT CTG CCT GAC	TCT CTC ACG CTC CCT CTC C	bp1/bp2	bp252/bp290	EH/VC SSU rRna

Specificity

Team Color	Forward primer	Reverse primer	Target site (e.g. bp 60), F	Target side (e.g., bp 300), R	Is bp # for VC or EH? Which gene?
Yellow
Blue	GATTCTGCCTGACGTAGATGC	CTGCTGGCACCAAACTTGC	10 bp	408 bp	E. hellem (AF118143)
Orange

W/F Section

Sensitivity

Team color	Forward (F) primer	Reverse (R) primer	Target site (e.g. bp 60), F	Target site (e.g., bp 300), R	Is bp # for VC or EH? Which gene?
Pink	ACC AGG TTG ATT CTG CTG ACG	TTGCGAGCATACTATCCCCAG	bp 1/2	bp 741/768	VC/EH SSU rRNA
Red	ACC AGG TTG ATT CTG CCT GAC	ATA TTA CCG CGG CTG CTG	bp 1/2	bp 380/421	VC/EH small subunit rRNA
Orange	CCA GGT TGA CTC TGC CTG ACG TCG
Yellow	CAC CAG GTT GAT TCT GCC TGA C	CCA TCC ATT TCC AAC GAC CAT G	1	947	EH

Specificity

Team color	Forward (F) primer	Reverse (R) primer	Target site (e.g. bp 60), F	Target site (e.g., bp 300), R	Is bp # for VC or EH? Which gene?
White
Blue	CAC CAG GTT GAT TCT GCC TGA C	CTG CTG GCA CCA GAC TTG C	1	426 for EH 386 for VC	Both
Silver	CAC CAG GTT GAT TCT GCC TGA C	TTC ACC ATA GAC ACT ACT GGA GC	1	441	EH
Purple	GTCTGGCTGTGTAGATAACTACG	CACTGTGAGACCTCGCATC	102	465	VC
Green	agattgctcccacgtccaag	caacgaccatgcaccactc	251	872	VC