Talk:20.109(S14):Diagnostic primer design (Day2): Difference between revisions
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==W/F Section== | |||
Revision as of 12:07, 12 February 2014
T/R Section
Sensitivity
| Team color | Forward (F) primer | Reverse (R) primer | Target site (e.g. bp 60), F | Target side (e.g., bp 300), R | Is bp # for VC or EH? Which gene? |
| Red | CAC CAG GTT GAT ACT GCC TGA C | CCT ATC CCG AAC CAA ACC CTG | bp 1 | bp 260 | EH, SSU rRNA |
| Green | |||||
| Pink | ACC AGG TTG ATT CTG CCT GAC | TCT CTC ACG CTC CCT CTC C | bp1/bp2 | bp252/bp290 | EH/VC SSU rRna |
Specificity
| Team Color | Forward primer | Reverse primer | Target site (e.g. bp 60), F | Target side (e.g., bp 300), R | Is bp # for VC or EH? Which gene? |
| Yellow | |||||
| Blue | GATTCTGCCTGACGTAGATGC | CTGCTGGCACCAAACTTGC | 10 bp | 408 bp | E. hellem (AF118143) |
| Orange |
