Talk:20.109(S14):Testing cell viability (Day3): Difference between revisions
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==T/R images== | ==T/R images== | ||
Green Team | Green Team (Blue Team: no yield from bead staining) | ||
[[Image:TR_Green_0uM_1_1.jpg | left |thumb|275px|TR Green 0 uM 1]] | [[Image:TR_Green_0uM_1_1.jpg | left |thumb|275px|TR Green 0 uM 1_1]] | ||
[[Image:TR_Green_0uM_1_2.jpg | right |thumb|275px|TR Green 0 uM 1_2]] | |||
[[Image:TR_Green_0uM_1_3.jpg | center |thumb|275px|TR Green 0 uM 1_3]] | |||
[[Image:TR_Green_0uM_1_4.jpg | left |thumb|275px|TR Green 0 uM 1_4]] | |||
[[Image:TR_Green_0uM_1_5.jpg | right |thumb|275px|TR Green 0 uM 1_5]] | |||
[[Image:TR_Green_0uM_2_1.jpg | center |thumb|275px|TR Green 0 uM 2_1]] | |||
[[Image:TR_Green_0uM_2_2.jpg | left |thumb|275px|TR Green 0 uM 2_2]] | |||
[[Image:TR_Green_0uM_2_3a.jpg | right |thumb|275px|TR Green 0 uM 2_3a]] | |||
[[Image:TR_Green_0uM_2_3b.jpg | center |thumb|275px|TR Green 0 uM 2_3b]] | |||
[[Image:TR_Green_0uM_2_4a.jpg | left |thumb|275px|TR Green 0 uM 2_4a]] | |||
[[Image:TR_Green_0uM_2_4b.jpg | right |thumb|275px|TR Green 0 uM 2_4b]] | |||
[[Image:TR_Green_0.5uM_1_1a.jpg | center |thumb|275px|TR Green 0.5 uM 1_1a]] | |||
[[Image:TR_Green_0.5uM_1_1b.jpg | left |thumb|275px|TR Green 0.5 uM 1_1b]] | |||
[[Image:TR_Green_0.5uM_1_2a.jpg | right |thumb|275px|TR Green 0.5 uM 1_2a]] | |||
[[Image:TR_Green_0.5uM_1_2b.jpg | center |thumb|275px|TR Green 0.5 uM 1_2b]] | |||
[[Image:TR_Green_0.5uM_1_3a.jpg | left |thumb|275px|TR Green 0.5 uM 1_3a]] | |||
[[Image:TR_Green_0.5uM_2_1a.jpg | right |thumb|275px|TR Green 0.5 uM 2_1a]] | |||
[[Image:TR_Green_0.5uM_2_1b.jpg | center |thumb|275px|TR Green 0.5 uM 2_1b]] | |||
[[Image:TR_Green_0.5uM_2_2a.jpg | left |thumb|275px|TR Green 0.5 uM 2_2a]] | |||
[[Image:TR_Green_0.5uM_2_2b.jpg | right |thumb|275px|TR Green 0.5 uM 2_2b]] | |||
[[Image:TR_Green_0.5uM_2_3a.jpg | center |thumb|275px|TR Green 0.5 uM 2_3a]] | |||
[[Image:TR_Green_0.5uM_2_3b.jpg | right |thumb|275px|TR Green 0.5 uM 2_3b]] | |||
<br style="clear:both;"/> | |||
Yellow/Pink teams | |||
[[Image:S14_TR_M3D3_YlwPnk_01.jpg|thumb|right|250px|TR Ylw/Pnk, 1.9mg/mL collagen 1]] | |||
[[Image:S14_TR_M3D3_YlwPnk_02.jpg|thumb|left|250px|TR Ylw/Pnk, 1.9mg/mL collagen 1]] | |||
[[Image:S14_TR_M3D3_YlwPnk_03.jpg|thumb|center|250px|TR Ylw/Pnk, 1.9mg/mL collagen 1]] | |||
[[Image:S14_TR_M3D3_YlwPnk_04.jpg|thumb|right|250px|TR Ylw/Pnk, 1.9mg/mL collagen 1]] | |||
[[Image:S14_TR_M3D3_YlwPnk_05.jpg|thumb|left|250px|TR Ylw/Pnk, 1.9mg/mL collagen 1]] | |||
[[Image:S14_TR_M3D3_YlwPnk_06.jpg|thumb|center|250px|TR Ylw/Pnk, 1.9mg/mL collagen 1]] | |||
[[Image:S14_TR_M3D3_YlwPnk_07.jpg|thumb|right|250px|TR Ylw/Pnk, 0mg/mL collagen 1]] | |||
[[Image:S14_TR_M3D3_YlwPnk_08.jpg|thumb|left|250px|TR Ylw/Pnk, 0mg/mL collagen 1]] | |||
[[Image:S14_TR_M3D3_YlwPnk_09.jpg|thumb|center|250px|TR Ylw/Pnk, 0mg/mL collagen 1]] | |||
[[Image:S14_TR_M3D3_YlwPnk_10.jpg|thumb|right|250px|TR Ylw/Pnk, 0mg/mL collagen 1]] | |||
[[Image:S14_TR_M3D3_YlwPnk_11.jpg|thumb|left|250px|TR Ylw/Pnk, 0mg/mL collagen 1]] | |||
[[Image:S14_TR_M3D3_YlwPnk_12.jpg|thumb|center|250px|TR Ylw/Pnk, 0mg/mL collagen 1]] | |||
[[Image:TR_PY_01_mgml_1.JPG|thumb|right|250px|TR Ylw/Pnk, 0.1mg/mL collagen 1]] | |||
[[Image:TR_PY_01_mgml_2.JPG|thumb|left|250px|TR Ylw/Pnk, 0.1mg/mL collagen 1]] | |||
[[Image:TR_PY_01_mgml_3.JPG|thumb|center|250px|TR Ylw/Pnk, 0.1mg/mL collagen 1]] | |||
[[Image:TR_PY_01_mgml_4.JPG|thumb|right|250px|TR Ylw/Pnk, 0.1mg/mL collagen 1]] | |||
[[Image:TR_PY_01_mgml_5.JPG|thumb|left|250px|TR Ylw/Pnk, 0.1mg/mL collagen 1]] | |||
[[Image:TR_PY_01_mgml_6.JPG|thumb|center|250px|TR Ylw/Pnk, 0.1mg/mL collagen 1]] | |||
[[Image:TR_PY_095_mgml_1.JPG|thumb|right|250px|TR Ylw/Pnk, 0.95mg/mL collagen 1]] | |||
[[Image:TR_PY_095_mgml_2.JPG|thumb|left|250px|TR Ylw/Pnk, 0.95mg/mL collagen 1]] | |||
[[Image:TR_PY_095_mgml_3.JPG|thumb|center|250px|TR Ylw/Pnk, 0.95mg/mL collagen 1]] | |||
[[Image:TR_PY_095_mgml_1b.JPG|thumb|right|250px|TR Ylw/Pnk, 0.95mg/mL collagen 1]] | |||
[[Image:TR_PY_095_mgml_2b.JPG|thumb|left|250px|TR Ylw/Pnk, 0.95mg/mL collagen 1]] | |||
[[Image:TR_PY_095_mgml_3b.JPG|thumb|center|250px|TR Ylw/Pnk, 0.95mg/mL collagen 1]] | |||
<br style="clear:both;"/> | |||
Red/Orange teams | |||
[[Image:TR_Red_Orange_0uM_3_1.jpg|thumb|right|250px|TR Red/Orange, 0uM ADAMTS-5]] | |||
[[Image:TR_Red_Orange_0uM_3_2.jpg|thumb|left|250px|TR Red/Orange, 0uM ADAMTS-5]] | |||
[[Image:TR_Red_Orange_0uM_3_4.jpg|thumb|center|250px|TR Red/Orange, 0uM ADAMTS-5]] | |||
[[Image:TR_Red_Orange_0uM_3_5.jpg|thumb|right|250px|TR Red/Orange, 0uM ADAMTS-5]] | |||
[[Image:TR_Red_Orange_0uM_3_6.jpg|thumb|left|250px|TR Red/Orange, 0uM ADAMTS-5]] | |||
[[Image:TR_Red_Orange_0uM_3_7.jpg|thumb|center|250px|TR Red/Orange, 0uM ADAMTS-5]] | |||
[[Image:TR_Red_Orange_2uM_1_1.jpg|thumb|right|250px|TR Red/Orange, 2uM ADAMTS-5]] | |||
[[Image:TR_Red_Orange_2uM_1_2.jpg|thumb|left|250px|TR Red/Orange, 2uM ADAMTS-5]] | |||
[[Image:TR_Red_Orange_2uM_1_6.jpg|thumb|center|250px|TR Red/Orange, 2uM ADAMTS-5]] | |||
[[Image:TR_Red_Orange_2uM_2_1.jpg|thumb|right|250px|TR Red/Orange, 2uM ADAMTS-5]] | |||
[[Image:TR_Red_Orange_2uM_2_2.jpg|thumb|left|250px|TR Red/Orange, 2uM ADAMTS-5]] | |||
[[Image:TR_Red_Orange_2uM_2_3.jpg|thumb|center|250px|TR Red/Orange, 2uM ADAMTS-5]] | |||
[[Image:TR02.JPG|thumb|right|250px|TR Red/Orange, 7uM ADAMTS-5]] | |||
[[Image:TR03.JPG|thumb|left|250px|TR Red/Orange, 7uM ADAMTS-5]] | |||
[[Image:TR04.JPG|thumb|center|250px|TR Red/Orange, 7uM ADAMTS-5]] | |||
[[Image:TR05.JPG|thumb|right|250px|TR Red/Orange, 7uM ADAMTS-5]] | |||
[[Image:TR06.JPG|thumb|left|250px|TR Red/Orange, 7uM ADAMTS-5]] | |||
[[Image:TR07.JPG|thumb|center|250px|TR Red/Orange, 7uM ADAMTS-5]] | |||
[[Image:TR08.JPG|thumb|right|250px|TR Red/Orange, 7uM ADAMTS-5]] | |||
[[Image:TR09.JPG|thumb|left|250px|TR Red/Orange, 10uM ADAMTS-5]] | |||
[[Image:TR10.JPG|thumb|center|250px|TR Red/Orange, 10uM ADAMTS-5]] | |||
[[Image:TR11.JPG|thumb|right|250px|TR Red/Orange, 10uM ADAMTS-5]] | |||
[[Image:TR12.JPG|thumb|left|250px|TR Red/Orange, 10uM ADAMTS-5]] | |||
[[Image:TR13.JPG|thumb|center|250px|TR Red/Orange, 7uM ADAMTS-5]] | |||
[[Image:TR14.JPG|thumb|right|250px|TR Red/Orange, 10uM ADAMTS-5]] | |||
[[Image:TR15.JPG|thumb|left|250px|TR Red/Orange, 10uM ADAMTS-5]] | |||
<br style="clear:both;"/> | |||
==W/F images== | ==W/F images== | ||
[[Image:WF_Org_surface_0.015g_hydroxyapetite.JPG|thumb|left|250px|WF Org/Red surface 0.015 g Hydroxyapetite]] | |||
[[Image:WF_YG_0_mg_mL_Collagen.JPG|thumb|left|250px|W/F Yel/Grn 0 mg/mL collagen]] | |||
[[Image:WF_YG_0.15_mg_mL_Collagen.JPG|thumb|left|250px|W/F Yel/Grn 0.15 mg/mL collagen]] | |||
[[Image:WF_PrpPinkB_surface1_10^-7_M_RA.JPG|thumb|center|250px|W/F Prp/Pink/Blue surface1 1e-7 M RA]] | |||
[[Image:WF_PrpPinkB__surface210^-7_M_RA.JPG|thumb|center|250px|W/F Prp/Pink/Blue surface2 1e-7 M RA]] | |||
[[Image:WF_PrpPinkB_core_10^-7_M_RA.JPG|thumb|center|250px|W/F Prp/Pink/Blue core 1e-7 M RA]] | |||
[[Image:WF_Blu_10^-5_RA_Chondro.JPG|thumb|center|250px|W/F Prp/Pink/Blue surface 1e-5 M RA Chondrocytes]] | |||
[[Image:WF_Blu_Control_MSC.JPG|thumb|center|250px|W/F Prp/Pink/Blue surface Control MSC]] | |||
[[Image:WF_Pink_High_RA_mostly_dead.JPG|thumb|center|250px|W/F Pink/Blue/Purple 1e-5 M RA]] | |||
[[Image:WF_Pink_High_RA_mostly_live.JPG|thumb|center|250px|W/F Pink/Blue/Purple 1e-5 M RA]] | |||
[[Image:WF_Red_core_0.0075g_ha.JPG|thumb|left|250px|WF Org/Red Core 0.0075 g Hydroxyapetite]] | |||
[[Image:WF_Org_core_0g_ha.JPG|thumb|left|250px|WF Org/Red Core 0 g Hydroxyapetite]] | |||
[[Image:WF_Red_core_0.03g_ha.JPG|thumb|left|250px|WF Org/Red Core 0.03 g Hydroxyapetite]] | |||
[[Image:WF_YG_250_1_Collagen.JPG|thumb|left|250px|W/F Yel/Grn 250 collagen]] | |||
[[Image:500_3.JPG|thumb|left|250px|W/F Yel/Grn 500 collagen]] | |||
[[Image:WF_YG_500_Dead_cells_Collagen.JPG|thumb|left|250px|W/F Yel/Grn 500 collagen]] | |||
[[Image:DSCN2716.JPG|thumb|left|250px|W/F Wht/Slv 250 collagen]] | |||
Latest revision as of 18:45, 5 May 2014
Please review section 6.1 of using OWW in 20.109 for a refresher on posting images.
T/R schedule
Staining protocol time is expected to be ~ 1 hour, leaving up to ~ 30 min blocks of microscope time for each group.
Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.
| Microscope time | Arrival time (at latest!) | Team color |
| 2:05-2:35 | 1:05 | Green |
| 2:35-3:05 | 1:25 | Yellow |
| 3:05-3:35 | 1:55 | Red |
| 3:35-4:05 | 2:25 | Orange |
| 4:05-4:35 | 2:55 | Blue |
| 4:35-5:05 | 3:25 | Pink |
W/F schedule
Staining protocol time is expected to be ~ 1 hour, leaving 20 min blocks of microscope time for each group.
Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.
| Microscope time | Arrival time (at latest!) | Team colour |
| 2:05-2:25 | 1:05 | orange |
| 2:25-2:45 | 1:15 | Yellow |
| 2:45-3:05 | 1:35 | Silver |
| 3:05-3:25 | 1:55 | Purple |
| 3:25-3:45 | 2:15 | Red |
| 3:45-4:05 | 2:35 | Green |
| 4:05-4:25 | 2:55 | Pink |
| 4:25-4:45 | 3:15 | White |
| 4:45-5:05 | 3:35 | Blue |
T/R images
Green Team (Blue Team: no yield from bead staining)
Yellow/Pink teams
Red/Orange teams
W/F images
