Talk:20.109(S14):Transcript-level analysis (Day6): Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→W/F) |
|||
Line 26: | Line 26: | ||
[[Image:S14_M3D5_TR_18S-melt.jpg|thumb|300px|right|T/R section, Melt curves for 18S samples]] | [[Image:S14_M3D5_TR_18S-melt.jpg|thumb|300px|right|T/R section, Melt curves for 18S samples]] | ||
[[Image:S14_M3D5_TR_CNII-melt.jpg|thumb|300px|left|T/R section, Melt curves for CN II samples]] | [[Image:S14_M3D5_TR_CNII-melt.jpg|thumb|300px|left|T/R section, Melt curves for CN II samples]] | ||
[[Image:S14_M3D5_TR_CNI-melt.jpg|thumb|300px|center|T/R section, Melt curves for CN | [[Image:S14_M3D5_TR_CNI-melt.jpg|thumb|300px|center|T/R section, Melt curves for CN I samples]] | ||
[[Image:S14_M3D5_TR_18S-noiseband.jpg|thumb|300px|right|T/R section, Amp curves for 18S samples]] | [[Image:S14_M3D5_TR_18S-noiseband.jpg|thumb|300px|right|T/R section, Amp curves for 18S samples]] | ||
[[Image:S14_M3D5_TR_CNII-noiseband.jpg|thumb|300px|left|T/R section, Amp curves for CN II samples]] | [[Image:S14_M3D5_TR_CNII-noiseband.jpg|thumb|300px|left|T/R section, Amp curves for CN II samples]] | ||
[[Image:S14_M3D5_TR_CNI-noiseband.jpg|thumb|300px|center|T/R section, Amp curves for CN | [[Image:S14_M3D5_TR_CNI-noiseband.jpg|thumb|300px|center|T/R section, Amp curves for CN I samples]] | ||
<br style="clear:both;"/> | <br style="clear:both;"/> | ||
Line 43: | Line 43: | ||
[[Image:S14_M3D5_WF_18S-melt.jpg|thumb|300px|right|W/F section, Melt curves for 18 samples]] | [[Image:S14_M3D5_WF_18S-melt.jpg|thumb|300px|right|W/F section, Melt curves for 18 samples]] | ||
[[Image:S14_M3D5_WF_CNII-melt.jpg|thumb|300px|left|W/F section, Melt curves for CN II samples]] | [[Image:S14_M3D5_WF_CNII-melt.jpg|thumb|300px|left|W/F section, Melt curves for CN II samples]] | ||
[[Image:S14_M3D5_WF_CNI-melt.jpg|thumb|300px|center|W/F section, Melt curves for CN | [[Image:S14_M3D5_WF_CNI-melt.jpg|thumb|300px|center|W/F section, Melt curves for CN I samples]] | ||
[[Image:S14_M3D5_WF_18S-noiseband.jpg|thumb|300px|right|W/F section, Amp curves for 18S samples]] | [[Image:S14_M3D5_WF_18S-noiseband.jpg|thumb|300px|right|W/F section, Amp curves for 18S samples]] | ||
[[Image:S14_M3D5_WF_CNII-noiseband.jpg|thumb|300px|left|W/F section, Amp curves for CN II samples]] | [[Image:S14_M3D5_WF_CNII-noiseband.jpg|thumb|300px|left|W/F section, Amp curves for CN II samples]] | ||
[[Image:S14_M3D5_WF_CNI-noiseband.jpg|thumb|300px|center|W/F section, Amp curves for CN | [[Image:S14_M3D5_WF_CNI-noiseband.jpg|thumb|300px|center|W/F section, Amp curves for CN I samples]] | ||
<br style="clear:both;"/> | <br style="clear:both;"/> | ||
Line 58: | Line 58: | ||
[[Image:S14_M3D5_WFxt_18S-melt.jpg|thumb|300px|right|W/F section, Melt curves for 18 samples]] | [[Image:S14_M3D5_WFxt_18S-melt.jpg|thumb|300px|right|W/F section, Melt curves for 18 samples]] | ||
[[Image:S14_M3D5_WFxt_CNII-melt.jpg|thumb|300px|left|W/F section, Melt curves for CN II samples]] | [[Image:S14_M3D5_WFxt_CNII-melt.jpg|thumb|300px|left|W/F section, Melt curves for CN II samples]] | ||
[[Image:S14_M3D5_WFxt_CNI-melt.jpg|thumb|300px|center|W/F section, Melt curves for CN | [[Image:S14_M3D5_WFxt_CNI-melt.jpg|thumb|300px|center|W/F section, Melt curves for CN I samples]] | ||
[[Image:S14_M3D5_WFxt_18S-noiseband.jpg|thumb|300px|right|W/F section, Amp curves for 18S samples]] | [[Image:S14_M3D5_WFxt_18S-noiseband.jpg|thumb|300px|right|W/F section, Amp curves for 18S samples]] | ||
[[Image:S14_M3D5_WFxt_CNII-noiseband.jpg|thumb|300px|left|W/F section, Amp curves for CN II samples]] | [[Image:S14_M3D5_WFxt_CNII-noiseband.jpg|thumb|300px|left|W/F section, Amp curves for CN II samples]] | ||
[[Image:S14_M3D5_WFxt_CNI-noiseband.jpg|thumb|300px|center|W/F section, Amp curves for CN | [[Image:S14_M3D5_WFxt_CNI-noiseband.jpg|thumb|300px|center|W/F section, Amp curves for CN I samples]] |
Revision as of 11:17, 6 May 2014
T/R pairings
W/F pairings
qPCR data
Files with CP values and additional notes
The Excel files below contains all of the section-wide data. Two different CP values are listed per sample. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set.
Media:20109-S14_TR_qPCR.xlsx
Media:20109-S14_WF_qPCR.xlsx
qPCR section-wide curves
T/R
W/F
Original run