Talk:20.109(S14):Transcript-level analysis (Day6): Difference between revisions
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==T/R pairings== | ==T/R pairings== | ||
[[Image:S14_research_disTR_pairs.jpg|thumb|left|400 px]]<br> | [[Image:S14_research_disTR_pairs.jpg|thumb|left|400 px]] | ||
<br style="clear:both;"/> | |||
==W/F pairings== | ==W/F pairings== | ||
[[Image:S14_M3D6-pairings.png|thumb|left|400px]] | [[Image:S14_M3D6-pairings.png|thumb|left|400px]] | ||
<br style="clear:both;"/> | |||
==qPCR data== | |||
===Files with CP values and additional notes=== | |||
The Excel files below contains all of the section-wide data. Two different CP values are listed per sample. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. | |||
[[Media:20109-S14_TR_qPCR.xlsx]] | |||
[[Media:20109-S14_WF_qPCR.xlsx]] | |||
==qPCR section-wide curves== | |||
===T/R=== | |||
[[Image:S14_M3D5_TR_raw.jpg|thumb|400px|left|T/R section, Raw amplification curves]] | |||
[[Image:S14_M3D5_TR_noiseband.jpg|thumb|400px|center|T/R section, Log-scale amplification curves with noise threshold]] | |||
[[Image:S14_M3D5_TR_18S-melt.jpg|thumb|300px|right|T/R section, Melt curves for 18S samples]] | |||
[[Image:S14_M3D5_TR_CNII-melt.jpg|thumb|300px|left|T/R section, Melt curves for CN II samples]] | |||
[[Image:S14_M3D5_TR_CNI-melt.jpg|thumb|300px|center|T/R section, Melt curves for CN II samples]] | |||
[[Image:S14_M3D5_TR_18S-noiseband.jpg|thumb|300px|right|T/R section, Amp curves for 18S samples]] | |||
[[Image:S14_M3D5_TR_CNII-noiseband.jpg|thumb|300px|left|T/R section, Amp curves for CN II samples]] | |||
[[Image:S14_M3D5_TR_CNI-noiseband.jpg|thumb|300px|center|T/R section, Amp curves for CN II samples]] | |||
<br style="clear:both;"/> | |||
===W/F=== | |||
[[Image:S14_M3D5_WF_raw.jpg|thumb|400px|left|W/F section, Raw amplification curves]] | |||
[[Image:S14_M3D5_WF_noiseband.jpg|thumb|400px|center|W/F section, Log-scale amplification curves with noise threshold]] | |||
[[Image:S14_M3D5_WF_18S-melt.jpg|thumb|300px|right|W/F section, Melt curves for 18 samples]] | |||
[[Image:S14_M3D5_WF_CNII-melt.jpg|thumb|300px|left|W/F section, Melt curves for CN II samples]] | |||
[[Image:S14_M3D5_WF_CNI-melt.jpg|thumb|300px|center|W/F section, Melt curves for CN II samples]] | |||
[[Image:S14_M3D5_WF_18S-noiseband.jpg|thumb|300px|right|W/F section, Amp curves for 18S samples]] | |||
[[Image:S14_M3D5_WF_CNII-noiseband.jpg|thumb|300px|left|W/F section, Amp curves for CN II samples]] | |||
[[Image:S14_M3D5_WF_CNI-noiseband.jpg|thumb|300px|center|W/F section, Amp curves for CN II samples]] | |||
<br style="clear:both;"/> |
Revision as of 09:19, 5 May 2014
T/R pairings
W/F pairings
qPCR data
Files with CP values and additional notes
The Excel files below contains all of the section-wide data. Two different CP values are listed per sample. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set.
Media:20109-S14_TR_qPCR.xlsx Media:20109-S14_WF_qPCR.xlsx
qPCR section-wide curves
T/R
W/F