Talk:20.109(S15):Begin Western protein analysis and choose system conditions (Day2)
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Please include both your section and your team color in one slot below. Across the class, be sure that each topology is tested by at least one group.
| Cut topology | Group 1 | Group 2 |
|---|---|---|
| Sticky ends, 5' overhangs | TR Blue | WF Yellow |
| Sticky ends, 3' overhangs | TR Prp | WF Pink |
| Blunt | TR Orange | WF Platinum |
| Hybrid: 5' sticky upstream, blunt downstream | WF Prp | WF Red |
| Hybrid: 3' sticky upstream, blunt downstream | TR Yellow | WF Green |
| Sticky ends with sequence mismatch | TR Green | WF orange |
| Sticky ends with sequence and topology mismatch |
