Talk:20.109(F07): Luciferase assays and RNA prep

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(T/R lab)
Line 82: Line 82:
| .048, .050
| .048, .050
|  
|  
-
|  
+
| ff 0.000,0.288 R 0.486,0.183
|  
|  
|
|
Line 90: Line 90:
| 2132, 17.75
| 2132, 17.75
|
|
-
|  
+
| ff 53.18,60.96 R 138.1,66.05
|  
|  
|
|
Line 98: Line 98:
|.056, .013
|.056, .013
|
|
-
|  
+
| ff 0.047,0.117 R 0.241,0.299
|
|
|
|
Line 106: Line 106:
| 158.3, 0.963
| 158.3, 0.963
|  
|  
-
|  
+
| ff 57.02,57.56 R 28.75,13.65
|
|
|
|
Line 114: Line 114:
| 1167, 3.408  
| 1167, 3.408  
|  
|  
-
|  
+
| ff 58.33,59.67 R 149.8,42.17
|
|
|
|
Line 122: Line 122:
| 202.9, 0.779
| 202.9, 0.779
|  
|  
-
|  
+
| ff 69.26,63.04 R 45.68,57.43
|
|
|
|
|}
|}
</center>
</center>

Revision as of 23:12, 21 October 2007

Luciferase Assay Data

Refer to table from first day of module to know what part of the Renilla luciferase gene was targeted by each group.
Please list values for each of your duplicate samples, separated by a comma.
Please calculate out any dilutions that you may have performed on your lysates so that the values listed here reflect the measurements for undiluted samples.
If there were any unusual things that are worth noting about your experiment, please note them below the table for your day of lab and "sign" your comment with your team color.

T/R lab

Sample Green Purple Red Blue Pink Yellow
No DNA .053, .054 11.47, 9.00 0.012, 0.0515
plasmid only 3826, 3299 29.50, 30.04 23.49, 682.95
siRNA only .063, .051 4.40, 10.40 0.006, 0.0615
plasmid+siRNA (validated) 136.4, 177.7 3.86, 4.05 19.56, 61.78
plasmid+siRNA (scrambled) 2184, 2819 32.29, 31.25 20.67, 603.95
plasmid+siRNA (experimental) 1170, 1209 15.58, 17.75 17.3, 182.4

W/F lab

Sample Green Purple Red Blue Pink Yellow
No DNA --. 0.042 .048, .050 ff 0.000,0.288 R 0.486,0.183
plasmid only 479.6, 206.2 2132, 17.75 ff 53.18,60.96 R 138.1,66.05
siRNA only 0.055, 0.058 .056, .013 ff 0.047,0.117 R 0.241,0.299
plasmid+siRNA (validated) 210.6, 130.5 158.3, 0.963 ff 57.02,57.56 R 28.75,13.65
plasmid+siRNA (scrambled) 1749, 1478 1167, 3.408 ff 58.33,59.67 R 149.8,42.17
plasmid+siRNA (experimental) 113, 107.1 202.9, 0.779 ff 69.26,63.04 R 45.68,57.43
Personal tools