Talk:20.109(F08): Mod 1 Day 6 Restriction map and tissue culture

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T/R Sample Gel

Here's a sample gel that was run with interpretable results. If your gel didn't work at all, or if none of your clones is right, then you can also include this image in your report to show your understanding of the experiment. The samples prepared exactly as you were directed to prepare yours.

sample diagnostic digest using BamHI and XhoI, M1D6(F08)


Lane Plasmid sample volume to load
1 pCX-NNX uncut 20 μL
2 cut with BamHI 20 μL
3 cut with XhoI 20 μL
4 cut with BamHI and XhoI 20 μL
5 1 kb marker 10 μL
6 candidate 1 uncut 20 μL
7 cut with BamHI 20 μL
8 cut with XhoI 20 μL
9 cut with BamHI and XhoI 20 μL
10 candidate 2 uncut 20 μL
11 cut with BamHI 20 μL
12 cut with XhoI 20 μL
13 cut with BamHI and XhoI 20 μL

W/F Data

Please fill in which two enzymes you used in the following table.

You can download your gel images below. If the images do not show up properly in your browser, just click on the place where the image is supposed to be, and it should be visible on a new page.

Please also see the T/R sample gel above if your gel experiment did not work as you had hoped - that is, if your bands are too faint to decipher, if one or both of your enzymes didn't cut at all, or if none of your clones gave the expected product. In this case, you should analyze both your own data and the sample data in your report.

Team colour Digest enzyme 1 Digest enzyme 2
Yellow BamHI XhoI
Green
Blue BamHI XhoI
Pink
Purple
W/F Yellow Group
W/F Yellow Group
W/F Green Group
W/F Green Group
W/F Blue Group
W/F Blue Group
W/F Pink Group
W/F Pink Group
W/F Purple Group
W/F Purple Group
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