Talk:20.109(F10): Mod 1 Day 3 Agarose gel electrophoresis

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(And here's the gel with your recovered fragments/backbones, ready for ligation. The brightest band on 1kb Ladder indicates 250ng of DNA)
(And here's the gel with your recovered fragments/backbones, ready for ligation. The brightest band on 1kb Ladder indicates 250ng of DNA)
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=== And here's the gel with your recovered fragments/backbones, ready for ligation. The brightest band on 1kb Ladder indicates 250ng of DNA===
=== And here's the gel with your recovered fragments/backbones, ready for ligation. The brightest band on 1kb Ladder indicates 250ng of DNA===
 +
Orange Team: In regards to your missing backbone, you might have too little backbone in the beginning or you've mistakenly discarded your backbone during gel purification process. Don't worry, you can certainly ask your neighbors or another group for some of their backbone to do ligation in the next lab. There should be plenty to use for everyone!
[[Image:100921Gel1.JPG]]
[[Image:100921Gel1.JPG]]

Revision as of 13:16, 22 September 2010

Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.

You can use these in your lab report if you so choose.



T/R lab

Red Orange Yellow Green
Blue Pink Purple Grey

And here's the gel with your recovered fragments/backbones, ready for ligation. The brightest band on 1kb Ladder indicates 250ng of DNA

Orange Team: In regards to your missing backbone, you might have too little backbone in the beginning or you've mistakenly discarded your backbone during gel purification process. Don't worry, you can certainly ask your neighbors or another group for some of their backbone to do ligation in the next lab. There should be plenty to use for everyone!

Image:100921Gel1.JPG

Image:100921Gel2.JPG

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