Talk:20.109(F10): Mod 2 Day 7 Readout of Protein

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(beta-gal Activity assay (Blue team))
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==Western Blot Results==
==Western Blot Results==
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*Each team will need to label their lanes.
*Each team will need to label their lanes.
*Team Pink did a second Western with interesting results.  They should add labels and a brief explanation of their work.
*Team Pink did a second Western with interesting results.  They should add labels and a brief explanation of their work.
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*Blue Team Western (below). Insert Labels.
[[Image:Blue_Western.jpg]]
[[Image:Blue_Western.jpg]]
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*Green Team Western (below). Insert Labels.
[[Image:Green_Western.jpg]]
[[Image:Green_Western.jpg]]
 +
*Pink Team Western (below). Insert Labels.
[[Image:Pink_Western.jpg]]
[[Image:Pink_Western.jpg]]
 +
*Orange Team Western (below). Insert Labels.
[[Image:Orange_Western.jpg]]
[[Image:Orange_Western.jpg]]
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*Silver Team Western (below). Insert Labels.
[[Image:Silver_Western.jpg]]
[[Image:Silver_Western.jpg]]
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*Yellow Team Western (below). Insert Labels.
[[Image:Yellow_Western.jpg]]
[[Image:Yellow_Western.jpg]]
 +
*Grape Team Western (below). Insert Labels.
[[Image:Grape_Western.jpg]]
[[Image:Grape_Western.jpg]]
 +
*Pink Team Extra Western (below). Insert Labels and explanation.
[[Image:Pink_2nd_Western.jpg]]
[[Image:Pink_2nd_Western.jpg]]

Revision as of 22:28, 7 November 2010

Western Blot Results

  • All team blots shown below (except red which chose not to develop theirs).
  • Each team will need to label their lanes.
  • Team Pink did a second Western with interesting results. They should add labels and a brief explanation of their work.
  • Blue Team Western (below). Insert Labels.

Image:Blue_Western.jpg

  • Green Team Western (below). Insert Labels.

Image:Green_Western.jpg

  • Pink Team Western (below). Insert Labels.

Image:Pink_Western.jpg

  • Orange Team Western (below). Insert Labels.

Image:Orange_Western.jpg

  • Silver Team Western (below). Insert Labels.

Image:Silver_Western.jpg

  • Yellow Team Western (below). Insert Labels.

Image:Yellow_Western.jpg

  • Grape Team Western (below). Insert Labels.

Image:Grape_Western.jpg

  • Pink Team Extra Western (below). Insert Labels and explanation.

Image:Pink_2nd_Western.jpg

beta-gal Activity assay (Blue team)

Error bars denote 1 stdev from the mean. Experiments were performed in triplicate.
Mut1 has an MGG mutation
Mut2 is unsequenced, but is probably loss-of-function
Also, L and D were mistakenly swapped (L bars denote dark trials, D bars denote light trials)
Image:Graph1.png

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