Talk:20.109(F11): Mod 1 Day 3 Agarose gel electrophoresis: Difference between revisions
| Line 69: | Line 69: | ||
=== And here's the gel with your recovered fragments/backbones, ready for ligation.=== | === And here's the gel with your recovered fragments/backbones, ready for ligation.=== | ||
<br><br> | <br><br> | ||
[[Image:WFGel1. | [[Image:WFGel1.jpg]] | ||
<br>Wednesday/Friday Gel 1:<br> | <br>Wednesday/Friday Gel 1:<br> | ||
Lane 1: Red Fragment<br>Lane 2: Red Backbone<br>Lane 3: Marker<br>Lane 4:Orange Fragment<br>Lane 5:Orange Backbone<br> | Lane 1: Red Fragment<br>Lane 2: Red Backbone<br>Lane 3: Marker<br>Lane 4:Orange Fragment<br>Lane 5:Orange Backbone<br> | ||
Revision as of 16:31, 23 September 2011
Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.
You can use these in your lab report if you so choose.
T/R lab
| Red | Orange | Yellow | Green |
| Blue | Purple | Pink | |
And here's the gel with your recovered fragments/backbones, ready for ligation.
Tuesday/Thursday Gel 1:
Lane 1: Red Fragment
Lane 2: Red Backbone
Lane 3:Orange Fragment
Lane 4:Orange Backbone
Lane 5: Marker
Lane 6: Yellow Fragment
Lane 7:Yellow Backbone
Lane 8:Green Fragment
Lane 9:Green Backbone
Lane 10:Blank
Tuesday/Thursday Gel 2:
Lane 1: Blue Fragment
Lane 2: Blue Backbone
Lane 3:Pink Fragment
Lane 4:Pink Backbone
Lane 5: Marker
Lane 6: Purple Fragment
Lane 7:Purple Backbone
Lane 8-10:Blank
W/F Lab
| Red | Orange | Yellow | Green |
| Blue | Purple | Pink | |
And here's the gel with your recovered fragments/backbones, ready for ligation.
Wednesday/Friday Gel 1:
Lane 1: Red Fragment
Lane 2: Red Backbone
Lane 3: Marker
Lane 4:Orange Fragment
Lane 5:Orange Backbone
Lane 6: Marker
Lane 7: Yellow Fragment
Lane 8:Yellow Backbone
Wednesday/Friday Gel 2:
Lane 1:Green Fragment
Lane 2:Blue Backbone
Lane 3:Marker
Lane 4: Blue Fragment
Lane 5: Blue Backbone
Lan3 6:Marker
Lane 7:Pink Fragment
Lane 8:Pink Backbone
Here are the pre-purification gels for reference
