Talk:20.109(F11): Mod 1 Day 3 Agarose gel electrophoresis

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(And here's the gel with your recovered fragments/backbones, ready for ligation.Note: All marker contain 15uL instead of 10.)
Current revision (18:34, 23 September 2011) (view source)
(And here's the gel with your recovered fragments/backbones, ready for ligation.)
 
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=== And here's the gel with your recovered fragments/backbones, ready for ligation.===
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=== And here's the gel with your recovered fragments/backbones, ready for ligation.<br>Note: All markers contain 15uL instead of 10.===
<br><br>
<br><br>
[[Image:WFGel1.jpg]]
[[Image:WFGel1.jpg]]
<br>Wednesday/Friday Gel 1:<br>
<br>Wednesday/Friday Gel 1:<br>
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Lane 1: Red Fragment<br>Lane 2: Red Backbone<br>Lane 3: Marker<br>Lane 4:Orange Fragment<br>Lane 5:Orange Backbone<br>
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Lane 1: Red Fragment<br>Lane 2: Red Backbone<br>Lane 3: Marker<br>Lane 4:Orange Fragment<br>Lane 5:Orange Backbone
<br>Lane 6: Marker<br>Lane 7: Yellow Fragment<br>Lane 8:Yellow Backbone
<br>Lane 6: Marker<br>Lane 7: Yellow Fragment<br>Lane 8:Yellow Backbone
<br style="clear:both;"/>
<br style="clear:both;"/>

Current revision

Contents

Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.

You can use these in your lab report if you so choose.



T/R lab

Red Orange Yellow Green
Blue Purple Pink

And here's the gel with your recovered fragments/backbones, ready for ligation.



Image:Th_Gel1.jpg
Tuesday/Thursday Gel 1:
Lane 1: Red Fragment
Lane 2: Red Backbone
Lane 3:Orange Fragment
Lane 4:Orange Backbone
Lane 5: Marker
Lane 6: Yellow Fragment
Lane 7:Yellow Backbone
Lane 8:Green Fragment
Lane 9:Green Backbone
Lane 10:Blank


Image:T-Th_Gel_2.jpg
Tuesday/Thursday Gel 2:
Lane 1: Blue Fragment
Lane 2: Blue Backbone
Lane 3:Pink Fragment
Lane 4:Pink Backbone
Lane 5: Marker
Lane 6: Purple Fragment
Lane 7:Purple Backbone
Lane 8-10:Blank

W/F Lab

Red Orange Yellow Green
Blue Purple Pink

And here's the gel with your recovered fragments/backbones, ready for ligation.
Note: All markers contain 15uL instead of 10.



Image:WFGel1.jpg
Wednesday/Friday Gel 1:
Lane 1: Red Fragment
Lane 2: Red Backbone
Lane 3: Marker
Lane 4:Orange Fragment
Lane 5:Orange Backbone
Lane 6: Marker
Lane 7: Yellow Fragment
Lane 8:Yellow Backbone


Image:WFGel2.jpg
Wednesday/Friday Gel 2:
Lane 1:Green Fragment
Lane 2:Blue Backbone
Lane 3:Marker
Lane 4: Blue Fragment
Lane 5: Blue Backbone
Lan3 6:Marker
Lane 7:Pink Fragment
Lane 8:Pink Backbone

Here are the pre-purification gels for reference

Blue Group
Blue Group
Green Group
Green Group
Orange Group
Orange Group
Pink Group
Pink Group
Red Group
Red Group
Yellow Group
Yellow Group
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