# Talk:20.109(F11): Mod 1 Day 7 FACS analysis

(Difference between revisions)
 Revision as of 16:38, 6 October 2011 (view source)← Previous diff Revision as of 10:40, 7 October 2011 (view source) (→W/F FACS schedule)Next diff → Line 94: Line 94: =W/F FACS schedule= =W/F FACS schedule= +
'''NOTE: data is %gated R2. If you'd like to know #s of cells assayed, please contact group directly.'''
{| border="1" {| border="1" |team color |team color |FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM) |FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM) + |mock data + |EGFP data + |delta 5 data + |delta 3 data + |delta5+delta3 + |delta5+delta3(Pme) + |delta5+delta3(Bam) + |extra experiment (specify below) |- |- |Red |Red - |III - 2:30-4PM + |III = 2:30-4PM + | + | + | + | + | + | + | + | |- |- |Orange |Orange |II = 1:30-3PM |II = 1:30-3PM + | + | + | + | + | + | + | + | |- |- |Yellow |Yellow |I = 12:30-2PM |I = 12:30-2PM + | + | + | + | + | + | + | |- |- |Green |Green - |II - 1:30-3PM + |II = 1:30-3PM + | + | + | + | + | + | + | + | |- |- |Blue |Blue - |I - 12:30-2PM + |I = 12:30-2PM + | + | + | + | + | + | + | + | |- |- |Pink |Pink - |III - 2:30-4PM + |III = 2:30-4PM - |- + | - |Purple + | - | + | - |- + | - |Silver + | + | + | | | |} |}

## Revision as of 10:40, 7 October 2011

Sign up for time on the FACS. Please arrive at the time you choose and then expect to spend 45 minutes in TC and 45 minutes at FACS. TWO or THREE teams should sign up for each time slot.

# T/R FACS schedule

NOTE: data is %gated R2. If you'd like to know #s of cells assayed, please contact group directly.
 team color FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM) mock data EGFP data delta 5 data delta 3 data delta5+delta3 delta5+delta3(Pme) delta5+delta3(Bam) extra experiment (specify below) Red I = 12:30-2PM 0.00 50.51 0.00 0.00 1.91 4.98 3.89 not done Orange III = 2:30-4PM 0.00 35.48 0.00 0.01 1.11 1.98 1.86 not done Yellow I = 12:30-2PM 0.00 45.28 0.00 0.00 0.89 3.33 2.59 Not done Green I = 12:30-2PM 0.00 58.5 0.01 0.01 1.61 4.33 4.92 ethidium bromide (100 ng/ml), 0.24 Blue II = 1:30-3PM 0.00 54.41 0.00 0.00 1.38 4.01 3.54 Not done Pink II = 1:30-3PM 0.00 46.04 0.01 0.00 1.19 3.97 2.69 Not done Purple II = 1:30-3PM 0.00 18.14 0.00 0.02 1.32 0.49 1.06 Not done

# W/F FACS schedule

NOTE: data is %gated R2. If you'd like to know #s of cells assayed, please contact group directly.
 team color FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM) mock data EGFP data delta 5 data delta 3 data delta5+delta3 delta5+delta3(Pme) delta5+delta3(Bam) extra experiment (specify below) Red III = 2:30-4PM Orange II = 1:30-3PM Yellow I = 12:30-2PM Green II = 1:30-3PM Blue I = 12:30-2PM Pink III = 2:30-4PM