Talk:20.109(F12): Mod 1 Day 3 Agarose gel electrophoresis

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(And here's the gel with your recovered fragments/backbones, ready for ligation.)
(And here's the gel with your recovered fragments/backbones, ready for ligation.)
Line 32: Line 32:
=== And here's the gel with your recovered fragments/backbones, ready for ligation.===
=== And here's the gel with your recovered fragments/backbones, ready for ligation.===
[[Image:TR Row1.jpg]]
[[Image:TR Row1.jpg]]
 +
 +
Row 1 legend is
 +
#Red backbone
 +
#Red fragment
 +
#1 kb ladder
 +
#Orange backbone
 +
#Orange fragment
 +
#Yellow backbone
 +
#Yellow fragment
 +
#1 kb ladder
 +
#Green backbone
 +
#Green fragment
=W/F Lab=
=W/F Lab=

Revision as of 18:17, 18 September 2012

Contents

Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.

You can use these in your assignments if you so choose.



T/R lab

Red Orange Yellow Green
Blue Purple Pink

And here's the gel with your recovered fragments/backbones, ready for ligation.

Image:TR Row1.jpg

Row 1 legend is

  1. Red backbone
  2. Red fragment
  3. 1 kb ladder
  4. Orange backbone
  5. Orange fragment
  6. Yellow backbone
  7. Yellow fragment
  8. 1 kb ladder
  9. Green backbone
  10. Green fragment

W/F Lab

Red Orange Yellow Green
Blue Purple Pink

And here's the gel with your recovered fragments/backbones, ready for ligation.

You can upload the pre-purification gels for reference here

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