Talk:20.109(F12): Mod 1 Day 3 Agarose gel electrophoresis: Difference between revisions
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|Green | |Green | ||
|- | |- | ||
| | |[[Image:KU000638.BMP|200px]] | ||
| | |[[Image:OrangeTRgel bkbinsert.BMP|200px]] | ||
| | |[[Image:KU000634.BMP|200px]] | ||
| | | | ||
|- | |- | ||
Line 33: | Line 33: | ||
[[Image:TR Row1.jpg]] | [[Image:TR Row1.jpg]] | ||
Row 1 legend | Row 1 legend: | ||
#Red backbone | #Red backbone | ||
#Red fragment | #Red fragment | ||
Line 44: | Line 44: | ||
#Green backbone | #Green backbone | ||
#Green fragment | #Green fragment | ||
[[Image:TR Row2.jpg]] | |||
Row 2 legend: | |||
#Blue backbone | |||
#Blue fragment | |||
#1 kb ladder | |||
#Pink backbone | |||
#Pink fragment | |||
#1 kb ladder | |||
#Purple backbone | |||
=W/F Lab= | =W/F Lab= | ||
Line 70: | Line 81: | ||
=== And here's the gel with your recovered fragments/backbones, ready for ligation.=== | === And here's the gel with your recovered fragments/backbones, ready for ligation.=== | ||
[[Image:WF Row1.jpg]] | |||
WF Legend: | |||
#Green backbone | |||
#Green fragment | |||
#1 kb ladder | |||
#Blue backbone | |||
#Blue fragment | |||
#Pink backbone | |||
#Pink fragment | |||
#1 kb ladder | |||
#Purple backbone | |||
#Purple fragment | |||
<br style="clear:both;"/> | |||
===You can upload the pre-purification gels for reference here=== | ===You can upload the pre-purification gels for reference here=== | ||
Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page! | |||
[[Image:F12_M1D3_WF-Grn.jpg|250px|thumb|left| Green Group]] | |||
[[Image:F12_M1D3_WF-Blu.jpg|250px|thumb|center| Blue Group]] | |||
[[Image:F12_M1D3_WF-Pnk.jpg|250px|thumb|left| Pink Group]] | |||
[[Image:F12_M1D3_WF-Prp.jpg|250px|thumb|center| Purple Group]] | |||
[[Image:F12_M1D3_TR_PNK.jpg|250px|thumb|center|PINK Group]] |
Latest revision as of 17:21, 25 September 2012
Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.
You can use these in your assignments if you so choose.
T/R lab
Red | Orange | Yellow | Green |
Blue | Purple | Pink | |
And here's the gel with your recovered fragments/backbones, ready for ligation.
Row 1 legend:
- Red backbone
- Red fragment
- 1 kb ladder
- Orange backbone
- Orange fragment
- Yellow backbone
- Yellow fragment
- 1 kb ladder
- Green backbone
- Green fragment
Row 2 legend:
- Blue backbone
- Blue fragment
- 1 kb ladder
- Pink backbone
- Pink fragment
- 1 kb ladder
- Purple backbone
W/F Lab
Red | Orange | Yellow | Green |
Blue | Purple | Pink | |
And here's the gel with your recovered fragments/backbones, ready for ligation.
WF Legend:
- Green backbone
- Green fragment
- 1 kb ladder
- Blue backbone
- Blue fragment
- Pink backbone
- Pink fragment
- 1 kb ladder
- Purple backbone
- Purple fragment
You can upload the pre-purification gels for reference here
Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!