Talk:20.109(F12): Mod 1 Day 3 Agarose gel electrophoresis

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Current revision (19:21, 25 September 2012) (view source)
(You can upload the pre-purification gels for reference here)
 
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[[Image:F12_M1D3_WF-Pnk.jpg|250px|thumb|left| Pink Group]]
[[Image:F12_M1D3_WF-Pnk.jpg|250px|thumb|left| Pink Group]]
[[Image:F12_M1D3_WF-Prp.jpg|250px|thumb|center| Purple Group]]
[[Image:F12_M1D3_WF-Prp.jpg|250px|thumb|center| Purple Group]]
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[[Image:F12_M1D3_TR_PNK.jpg|250px|thumb|center|PINK Group]]

Current revision

Contents

Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.

You can use these in your assignments if you so choose.



T/R lab

Red Orange Yellow Green
Blue Purple Pink

And here's the gel with your recovered fragments/backbones, ready for ligation.

Image:TR Row1.jpg

Row 1 legend:

  1. Red backbone
  2. Red fragment
  3. 1 kb ladder
  4. Orange backbone
  5. Orange fragment
  6. Yellow backbone
  7. Yellow fragment
  8. 1 kb ladder
  9. Green backbone
  10. Green fragment

Image:TR Row2.jpg

Row 2 legend:

  1. Blue backbone
  2. Blue fragment
  3. 1 kb ladder
  4. Pink backbone
  5. Pink fragment
  6. 1 kb ladder
  7. Purple backbone

W/F Lab

Red Orange Yellow Green
Blue Purple Pink

And here's the gel with your recovered fragments/backbones, ready for ligation.

Image:WF Row1.jpg

WF Legend:

  1. Green backbone
  2. Green fragment
  3. 1 kb ladder
  4. Blue backbone
  5. Blue fragment
  6. Pink backbone
  7. Pink fragment
  8. 1 kb ladder
  9. Purple backbone
  10. Purple fragment


You can upload the pre-purification gels for reference here

Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!

Green Group
Green Group
Blue Group
Blue Group
Error creating thumbnail: Invalid thumbnail parameters
Pink Group
Purple Group
Purple Group
PINK Group
PINK Group
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