Talk:20.109(F12): Mod 2 Day 5 Assessing re-tuned system

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T/R Mutant Miller Assay

Please enter the results of your B-gal assay and your mutation (after sequencing)

Team Color Mutant 1 B-Gal Average (Dark) Mutant 1 B-Gal Average (Light) Mutant 1 T541X; X = Mutant 2 B-Gal Average (Dark) Mutant 2 B-Gal Average (Light) Mutant 2 T541X; X = Wild Type System B-Gal (Dark) Wild Type System B-Gal (Light) H243A Mutant B-Gal (Dark) H243A Mutant B-Gal (Light)
Red 1324.65 1054.65 Ala 1268.2 1106.4 Leu 944.2 330.8 71.95 66
Orange 1077.76 351.56 1228.68 458.33 1105.34 418.94 65.42 63.91
Yellow 1552.5 494.95 1711.65 458.85 1065.9 389.2 107.9 102.0
Green 1287.79 531.26 N/A (WT) 749.18 390.12 N/A (WT) 1133.14 359.73 52.38 49.77
Blue 1149.27 244.80 None 1208.32 532.60 None 1573.52 537.10 91.79 80.99
Pink 1597.37 528.91 Gly 1037.31 237.98 WT 1000.73 323.69 49.52 48.48
Purple 1208.35 1018.45 Leu 1328.9 190.95 WT 2192.9 322.85 43.15 38.80

W/F Mutant Miller Assay

Please enter the results of your B-gal assay and your mutation (after sequencing)

Team Color Mutant 1 B-Gal Average (Dark) Mutant 1 B-Gal Average (Light) Mutant 1 T541X; X = Mutant 2 B-Gal Average (Dark) Mutant 2 B-Gal Average (Light) Mutant 2 T541X; X = Wild Type System B-Gal (Dark) Wild Type System B-Gal (Light) H243A Mutant B-Gal (Dark) H243A Mutant B-Gal (Light)
Green
Blue 1160 349 Gly 1463 1019 Asn 1500 627 92 112
Pink 2328.25 1928.66 -- -- 1099.13 414.85 54.54 45.27
Purple 1417.88 1409.76 1450.07 454.78 1099.13 414.85 54.54 45.27
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