Talk:20.109(F12): Mod 2 Day 5 Assessing re-tuned system

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(→T/R Mutant Miller Assay)
Current revision (08:02, 12 November 2012) (view source)
(→W/F Mutant Miller Assay)
 
(13 intermediate revisions not shown.)
Line 17: Line 17:
|1324.65
|1324.65
|1054.65
|1054.65
-
|
+
|Ala
|1268.2
|1268.2
|1106.4
|1106.4
-
|
+
|Leu
|944.2
|944.2
|330.8
|330.8
Line 41: Line 41:
|1552.5
|1552.5
|494.95
|494.95
-
|
+
|WT
|1711.65
|1711.65
|458.85
|458.85
-
|
+
|WT
|1065.9
|1065.9
|389.2
|389.2
Line 53: Line 53:
|1287.79
|1287.79
|531.26
|531.26
-
|
+
|N/A (WT)
|749.18
|749.18
|390.12
|390.12
-
|
+
|N/A (WT)
|1133.14
|1133.14
|359.73
|359.73
Line 65: Line 65:
|1149.27
|1149.27
|244.80
|244.80
-
|
+
|None
|1208.32
|1208.32
|532.60
|532.60
-
|
+
|None
|1573.52
|1573.52
|537.10
|537.10
Line 77: Line 77:
|1597.37
|1597.37
|528.91
|528.91
-
|
+
|Gly
|1037.31
|1037.31
|237.98
|237.98
-
|
+
|WT
|1000.73
|1000.73
|323.69
|323.69
Line 89: Line 89:
|1208.35
|1208.35
|1018.45
|1018.45
-
|
+
|Leu
|1328.9
|1328.9
|190.95
|190.95
-
|
+
|WT
|2192.9
|2192.9
|322.85
|322.85
Line 115: Line 115:
|-
|-
|Green
|Green
-
|
+
|566.681
-
|
+
|445.824
-
|
+
|Leu
-
|
+
|405.025
-
|
+
|209.527
-
|
+
|His
-
|
+
|830.317
-
|
+
|390.682
-
|
+
|56.498
-
|
+
|55.302
|-
|-
|Blue
|Blue
-
|
+
|1160
-
|
+
|349
-
|
+
|Gly
-
|
+
|1463
-
|
+
|1019
-
|
+
|Asn
-
|
+
|1500
-
|
+
|627
-
|
+
|92
-
|
+
|112
|-
|-
|Pink
|Pink
 +
|2328.25
 +
|1928.66
|
|
 +
| --
 +
| --
|
|
-
|
+
|1099.13
-
|
+
|414.85
-
|
+
|54.54
-
|
+
|45.27
-
|
+
-
|
+
-
|
+
-
|
+
|-
|-
|Purple
|Purple
 +
|1417.88
 +
|1409.76
|
|
 +
|1450.07
 +
|454.78
|
|
-
|
+
|1099.13
-
|
+
|414.85
-
|
+
|54.54
-
|
+
|45.27
-
|
+
-
|
+
-
|
+
-
|
+
|}
|}

Current revision

T/R Mutant Miller Assay

Please enter the results of your B-gal assay and your mutation (after sequencing)

Team Color Mutant 1 B-Gal Average (Dark) Mutant 1 B-Gal Average (Light) Mutant 1 T541X; X = Mutant 2 B-Gal Average (Dark) Mutant 2 B-Gal Average (Light) Mutant 2 T541X; X = Wild Type System B-Gal (Dark) Wild Type System B-Gal (Light) H243A Mutant B-Gal (Dark) H243A Mutant B-Gal (Light)
Red 1324.65 1054.65 Ala 1268.2 1106.4 Leu 944.2 330.8 71.95 66
Orange 1077.76 351.56 1228.68 458.33 1105.34 418.94 65.42 63.91
Yellow 1552.5 494.95 WT 1711.65 458.85 WT 1065.9 389.2 107.9 102.0
Green 1287.79 531.26 N/A (WT) 749.18 390.12 N/A (WT) 1133.14 359.73 52.38 49.77
Blue 1149.27 244.80 None 1208.32 532.60 None 1573.52 537.10 91.79 80.99
Pink 1597.37 528.91 Gly 1037.31 237.98 WT 1000.73 323.69 49.52 48.48
Purple 1208.35 1018.45 Leu 1328.9 190.95 WT 2192.9 322.85 43.15 38.80

W/F Mutant Miller Assay

Please enter the results of your B-gal assay and your mutation (after sequencing)

Team Color Mutant 1 B-Gal Average (Dark) Mutant 1 B-Gal Average (Light) Mutant 1 T541X; X = Mutant 2 B-Gal Average (Dark) Mutant 2 B-Gal Average (Light) Mutant 2 T541X; X = Wild Type System B-Gal (Dark) Wild Type System B-Gal (Light) H243A Mutant B-Gal (Dark) H243A Mutant B-Gal (Light)
Green 566.681 445.824 Leu 405.025 209.527 His 830.317 390.682 56.498 55.302
Blue 1160 349 Gly 1463 1019 Asn 1500 627 92 112
Pink 2328.25 1928.66 -- -- 1099.13 414.85 54.54 45.27
Purple 1417.88 1409.76 1450.07 454.78 1099.13 414.85 54.54 45.27
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