Talk:20.109(F13): Mod 1 Day 3 Agarose gel electrophoresis: Difference between revisions
Line 47: | Line 47: | ||
[[Image:F13_M1D3_WF-recovery.jpg|550px|thumb|center| W/F Recovery Gel]] | [[Image:F13_M1D3_WF-recovery.jpg|550px|thumb|center| W/F Recovery Gel]] | ||
Top Gel (numbers refer to lanes): | |||
*1-2 Red | |||
*3-4 Orange | |||
*5 MW | |||
*6-7 Yellow | |||
*8-9 Green | |||
Bottom Gel (numbers refer to lanes): | |||
*1-2 Blue | |||
*3-4 Pink | |||
*5 MW | |||
*6-7 Purple | |||
*8-9 White | |||
Note: We loaded all of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg). | |||
===Pre-purification gels (for reference/posterity)=== | ===Pre-purification gels (for reference/posterity)=== |
Revision as of 15:48, 18 September 2013
Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.
T/R lab
Recovery gel
Top Gel (numbers refer to lanes):
- 1-2 Pink
- 3-4 Purple
- 5 MW
- 6-7 White
- 8-9 Blue
Bottom Gel (numbers refer to lanes):
- 1-2 Orange
- 3-4 Yellow
- 5 MW
- 6-7 Green
- 8-9 Platinum
- 10 Red
Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg).
Pre-purification gels (for reference/posterity)
Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!
W/F lab
Recovery gel
Top Gel (numbers refer to lanes):
- 1-2 Red
- 3-4 Orange
- 5 MW
- 6-7 Yellow
- 8-9 Green
Bottom Gel (numbers refer to lanes):
- 1-2 Blue
- 3-4 Pink
- 5 MW
- 6-7 Purple
- 8-9 White
Note: We loaded all of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg).
Pre-purification gels (for reference/posterity)
Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!